The HP0256 gene product is involved in motility and cell envelope architecture of Helicobacter pylori

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dc.contributor.authorDouillard, François P.
dc.contributor.authorRyan, Kieran A.
dc.contributor.authorLane, Michael C.
dc.contributor.authorCaly, Delphine L.
dc.contributor.authorMoore, Stanley A.
dc.contributor.authorPenn, Charles W.
dc.contributor.authorHinds, Jason
dc.contributor.authorO'Toole, Paul W.
dc.contributor.funderScience Foundation Irelanden
dc.contributor.funderNatural Sciences and Engineering Research Council of Canadaen
dc.contributor.funderWellcome Trust, United Kingdomen
dc.date.accessioned2012-11-12T13:25:14Z
dc.date.available2012-11-12T13:25:14Z
dc.date.copyright2010
dc.date.issued2010-04-08
dc.description.abstractBackground: Helicobacter pylori is the causative agent for gastritis, and peptic and duodenal ulcers. The bacterium displays 5-6 polar sheathed flagella that are essential for colonisation and persistence in the gastric mucosa. The biochemistry and genetics of flagellar biogenesis in H. pylori has not been fully elucidated. Bioinformatics analysis suggested that the gene HP0256, annotated as hypothetical, was a FliJ homologue. In Salmonella, FliJ is a chaperone escort protein for FlgN and FliT, two proteins that themselves display chaperone activity for components of the hook, the rod and the filament. Results: Ablation of the HP0256 gene in H. pylori significantly reduced motility. However, flagellin and hook protein synthesis was not affected in the HP0256 mutant. Transmission electron transmission microscopy revealed that the HP0256 mutant cells displayed a normal flagellum configuration, suggesting that HP0256 was not essential for assembly and polar localisation of the flagella in the cell. Interestingly, whole genome microarrays of an HP0256 mutant revealed transcriptional changes in a number of genes associated with the flagellar regulon and the cell envelope, such as outer membrane proteins and adhesins. Consistent with the array data, lack of the HP0256 gene significantly reduced adhesion and the inflammatory response in host cells. Conclusions: We conclude that HP0256 is not a functional counterpart of FliJ in H. pylori. However, it is required for full motility and it is involved, possibly indirectly, in expression of outer membrane proteins and adhesins involved in pathogenesis and adhesion.en
dc.description.sponsorshipScience Foundation Ireland (CSET); Science Foundation Ireland (Research Frontiers Programme); Natural Sciences and Engineering Research Council of Canada (Discovery Grant RGPIN262138-05)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationDouillard, F.P., K. A. Ryan, M. C. Lane, D.L. Caly, S. A. Moore, C. W. Penn, J. Hinds, and P. W. O’Toole. (2010). The HP0256 gene product is involved in motility and cell envelope architecture of Helicobacter pylori. BMC Microbiology, 10:106 doi:10.1186/1471-2180-10-106en
dc.identifier.doi10.1186/1471-2180-10-106
dc.identifier.issn1471-2180
dc.identifier.issued10en
dc.identifier.journaltitleBMC Microbiologyen
dc.identifier.startpage106en
dc.identifier.urihttps://hdl.handle.net/10468/757
dc.language.isoenen
dc.publisherBioMed Centralen
dc.relation.urihttp://www.biomedcentral.com/1471-2180/10/106
dc.rights© 2010 Douillard et al; licensee BioMed Central Ltd.en
dc.rights.urihttp://creativecommons.org/licenses/by/2.0en
dc.subjectHelicobacter pylorien
dc.subjectHP0256en
dc.subjectFliJen
dc.subjectGastritisen
dc.subjectUlceren
dc.subjectSalmonellaen
dc.subjectCell envelope architectureen
dc.titleThe HP0256 gene product is involved in motility and cell envelope architecture of Helicobacter pylorien
dc.typeArticle (peer-reviewed)en
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