Bifidobacterium breve UCC2003 induces a distinct global transcriptomic program in neonatal murine intestinal epithelial cells

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dc.contributor.authorKiu, Raymond
dc.contributor.authorTreveil, Agatha
dc.contributor.authorHarnisch, Lukas C.
dc.contributor.authorCaim, Shabhonam
dc.contributor.authorLeclaire, Charlotte
dc.contributor.authorvan Sinderen, Douwe
dc.contributor.authorKorcsmaros, Tamas
dc.contributor.authorHall, Lindsay J.
dc.contributor.funderNorwich Bioscience Institutes (NBI) Computing infrastructure for Science (CiS) group
dc.contributor.funderWellcome Trust
dc.contributor.funderGut Microbes and Health
dc.contributor.funderGenomics for Food security
dc.contributor.funderNorwich Research Park Biosciences
dc.contributor.funderScience Foundation Ireland
dc.date.accessioned2023-11-08T11:12:01Z
dc.date.available2023-11-08T10:00:35Zen
dc.date.available2023-11-08T11:12:01Z
dc.date.issued2020-07en
dc.date.updated2023-11-08T10:00:38Zen
dc.description.abstractThe underlying health-driving mechanisms of Bifidobacterium during early life are not well understood, particularly how this microbiota member may modulate the intestinal barrier via programming of intestinal epithelial cells (IECs). We investigated the impact of Bifidobacterium breve UCC2003 on the transcriptome of neonatal murine IECs. Small IECs from two-week-old neonatal mice administered B. breve UCC2003 or PBS (control) were subjected to global RNA sequencing, and differentially expressed genes, pathways, and affected cell types were determined. We observed extensive regulation of the IEC transcriptome with ∼4,000 genes significantly up-regulated, including key genes linked with epithelial barrier function. Enrichment of cell differentiation pathways was observed, along with an overrepresentation of stem cell marker genes, indicating an increase in the regenerative potential of the epithelial layer. In conclusion, B. breve UCC2003 plays a central role in driving intestinal epithelium homeostatic development during early life and suggests future avenues for next-stage clinical studies.
dc.description.sponsorshipWellcome Trust (Investigator award (100974/C/13/Z)); Gut Microbes and Health (BB/R012490/1, BBS/E/F/000PR10353 and BBS/E/F/000PR10355); Genomics for Food security(BBSRC (BB/CSP17270/1)); Norwich Research Park Biosciences (Doctoral Training Partnership Grant BB/M011216/1)
dc.description.statusPeer revieweden
dc.description.versionPublished Version
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid101336
dc.identifier.citationKiu, R., Treveil, A., Harnisch, L.C., Caim, S., Leclaire, C., Van Sinderen, D., Korcsmaros, T. and Hall, L.J. (2020) ‘Bifidobacterium breve ucc2003 induces a distinct global transcriptomic program in neonatal murine intestinal epithelial cells’, iScience, 23(7), 101336 (26pp). doi: 10.1016/j.isci.2020.101336
dc.identifier.doi10.1016/j.isci.2020.101336en
dc.identifier.endpage26
dc.identifier.issn2589-0042
dc.identifier.issued7
dc.identifier.journaltitleiScience
dc.identifier.startpage1
dc.identifier.urihttps://hdl.handle.net/10468/15205
dc.identifier.volume23
dc.language.isoenen
dc.publisherElsevier
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2273/IE/Alimentary Pharmabiotic Centre (APC) - Interfacing Food & Medicine/
dc.rights© 2020 The Author(s). This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectMicrobiology
dc.subjectMicrobiome
dc.subjectTranscriptomics
dc.titleBifidobacterium breve UCC2003 induces a distinct global transcriptomic program in neonatal murine intestinal epithelial cells
dc.typeArticle (peer-reviewed)
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