Lactococcus lactis is capable of improving the riboflavin status in deficient rats

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dc.contributor.authorLeBlanc, Jean Guy
dc.contributor.authorBurgess, Catherine M.
dc.contributor.authorSesma, Fernando
dc.contributor.authorde Giori, Graciela Savoy
dc.contributor.authorvan Sinderen, Douwe
dc.contributor.funderScience Foundation Irelanden
dc.contributor.funderEuropean Commissionen
dc.contributor.funderConsejo Nacional de Investigaciones Cientificas y Tecnicas, Argentinaen
dc.contributor.funderConsejo de Investigaciones de la Universidad Nacional de Tucumán, Argentinaen
dc.date.accessioned2012-12-12T10:16:40Z
dc.date.available2012-12-12T10:16:40Z
dc.date.copyright2005
dc.date.issued2005
dc.description.abstractLactococcus lactis is a commonly used starter strain that can be converted from a vitamin B2 consumer into a vitamin B2 'factory' by over-expressing its riboflavin biosynthesis genes. The present study was conducted to assess in a rat bioassay the response of riboflavin produced by GM or native lactic acid bacteria (LAB). The riboflavin-producing strains were able to eliminate most physiological manifestations of ariboflavinosis such as stunted growth, elevated erythrocyte glutathione reductase activation coefficient values and hepatomegalia that were observed using a riboflavin depletion–repletion model. Riboflavin status and growth rates were greatly improved when the depleted rats were fed with cultures of L. lactis that overproduced this vitamin whereas the native strain did not show the same effect. The present study is the first animal trial with food containing living bacteria that were engineered to overproduce riboflavin. These results pave the way for analysing the effect of similar riboflavin-overproducing LAB in human trials.en
dc.description.sponsorshipScience Foundation Ireland (SFI-CSET); European Commission (QLK1-CT-2000-01 376 (www.nutracells.com))en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationJean Guy LeBlanc, Catherine Burgess, Fernando Sesma, Graciela Savoy de Giori and Douwe van Sinderen (2005). Lactococcus lactis is capable of improving the riboflavin status in deficient rats. British Journal of Nutrition, 94, pp 262-267. doi: 10.1079/BJN20051473en
dc.identifier.doi10.1079/BJN20051473
dc.identifier.endpage267en
dc.identifier.issn0007-1145
dc.identifier.issn1475-2662
dc.identifier.journaltitleBritish Journal of Nutritionen
dc.identifier.startpage262en
dc.identifier.urihttps://hdl.handle.net/10468/843
dc.identifier.volume94en
dc.language.isoenen
dc.publisherCambridge University Pressen
dc.relation.urihttp://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=920456
dc.rights©The Authors 2005en
dc.rights.urihttp://journals.cambridge.org/action/stream?pageId=4088&level=2#4408en
dc.subjectRiboflavinen
dc.subjectLactic acid bacteriaen
dc.subjectAriboflavinosisen
dc.subjectGenetically modified micro-organismsen
dc.subjectLactococcus lactisen
dc.titleLactococcus lactis is capable of improving the riboflavin status in deficient ratsen
dc.typeArticle (peer-reviewed)en
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