Cholestasis induced by bile duct ligation promotes changes in the intestinal microbiome in mice

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dc.contributor.authorCabrera-Rubio, Raul
dc.contributor.authorPatterson, Angela M.
dc.contributor.authorCotter, Paul D.
dc.contributor.authorBeraza, Naiara
dc.contributor.funderBiotechnology and Biological Sciences Research Councilen
dc.date.accessioned2019-12-05T10:11:44Z
dc.date.available2019-12-05T10:11:44Z
dc.date.issued2019-08-23
dc.description.abstractIncreasing evidence point to the relevance of intestinal disfunction and changes in the microbiome composition during chronic liver disease. More specifically, recent studies have highlighted that cholestatic diseases associate with a reduction in the microbiome diversity in patients. Still, the dynamics of the changes in the microbiome composition observed, as well as their implication in contributing to the pathogenesis of this disease remain largely undefined. Hence, experimental mouse models resembling the human pathogenesis are crucial to move forward our understanding on the mechanisms underpinning cholestatic disease and to enable the development of effective therapeutics. Our results show that the bile duct ligation (BDL) experimental model of cholestasis leads to rapid and significant changes in the microbiome diversity, with more than 100 OTUs being significantly different in faecal samples obtained from WT mice at 3 days and 7 days after BDL when compared to control animals. Changes in the microbial composition in mice after BDL included the enrichment of Akkermansia, Prevotella, Bacteroides and unclassified Ruminococcaceae in parallel with a drastic reduction of the presence of Faecalibacterium prausnitzii. In conclusion, our results support that bile duct ligation induces changes in the microbiome that partly resemble the gut microbial changes observed during human cholestatic disease.en
dc.description.sponsorshipBiological Sciences Research Council (BB/J004529/1, BBS/E/F/00044509, BB/R012490/1, BBS/E/F/000PR10355, BB/CCG1860/1)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid12324en
dc.identifier.citationCabrera-Rubio, R., Patterson, A. M., Cotter, P. D. and Beraza, N. (2019) 'Cholestasis induced by bile duct ligation promotes changes in the intestinal microbiome in mice', Scientific Reports, 9(1), 12324. (10pp.) doi: 10.1038/s41598-019-48784-zen
dc.identifier.doi10.1038/s41598-019-48784-zen
dc.identifier.eissn2045-2322
dc.identifier.endpage10en
dc.identifier.issued1en
dc.identifier.journaltitleScientific Reportsen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/9335
dc.identifier.volume9en
dc.language.isoenen
dc.publisherNature Publishing Groupen
dc.rights© The Author(s) 2019. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectCholestasisen
dc.subjectMicrobiologyen
dc.subjectBile ducten
dc.subjectIntestinal microbiomeen
dc.titleCholestasis induced by bile duct ligation promotes changes in the intestinal microbiome in miceen
dc.typeArticle (peer-reviewed)en
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