Evidence for plasmid-mediated salt tolerance in the human gut microbiome and potential mechanisms

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dc.contributor.authorBroaders, Eileen
dc.contributor.authorO'Brien, Ciarán
dc.contributor.authorGahan, Cormac G. M.
dc.contributor.authorMarchesi, Julian R.
dc.contributor.funderScience Foundation Irelanden
dc.date.accessioned2019-11-01T07:35:35Z
dc.date.available2019-11-01T07:35:35Z
dc.date.issued2016-02-04
dc.description.abstractThe human gut microbiome is critical to health and wellbeing. It hosts a complex ecosystem comprising a multitude of bacterial species, which contributes functionality that would otherwise be absent from the host. Transient and commensal bacteria in the gut must withstand many stresses. The influence of mobile genetic elements such as plasmids in stress adaptation within the ecosystem is poorly understood. Using a mobilomic approach we found evidence for plasmid-mediated osmotolerance as a phenotype amongst the Proteobacteria in healthy faecal slurries. A transconjugant carrying multiple plasmids acquired from healthy faecal slurry demonstrated increased osmotolerance in the presence of metal salts, particularly potassium chloride, which was not evident in the recipient. Pyrosequencing and analysis of the total plasmid DNA demonstrated the presence of plasmid-borne osmotolerance systems (including KdpD and H-NS) which may be linked to the observed phenotype. This is the first report of a transferable osmotolerance phenotype in gut commensals and may have implications for the transfer of osmotolerance in other niches.en
dc.description.sponsorshipScience Foundation Ireland (Centres for Engineering Science and Technology program)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleidfiw019en
dc.identifier.citationBroaders, E., O’Brien, C., Gahan, C. G. M. and Marchesi, J. R. (2016) 'Evidence for plasmid-mediated salt tolerance in the human gut microbiome and potential mechanisms', FEMS Microbiology Ecology, 92(3), fiw019. (8pp.) DOI: 10.1093/femsec/fiw019en
dc.identifier.doi10.1093/femsec/fiw019en
dc.identifier.eissn1574-6941
dc.identifier.endpage8en
dc.identifier.issn0168-6496
dc.identifier.issued3en
dc.identifier.journaltitleFEMS Microbiology Ecologyen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/8935
dc.identifier.volume92en
dc.language.isoenen
dc.publisherOxford University Pressen
dc.relation.urihttps://academic.oup.com/femsec/article/92/3/fiw019/2470104
dc.rights©FEMS 2016. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectGuten
dc.subjectMicrobiomeen
dc.subjectMobile genetic elementsen
dc.subjectOsmotoleranceen
dc.titleEvidence for plasmid-mediated salt tolerance in the human gut microbiome and potential mechanismsen
dc.typeArticle (peer-reviewed)en
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