Genomic and ecogenomic characterisation of Proteus mirabilis bacteriophage

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dc.contributor.authorAlves, Diana R.
dc.contributor.authorNzakizwanayo, Jonathan
dc.contributor.authorDedi, Cinzia
dc.contributor.authorOlympiou, Chara
dc.contributor.authorHanin, Aurélie
dc.contributor.authorKot, Witold
dc.contributor.authorHansen, Lars
dc.contributor.authorLametsch, Rene
dc.contributor.authorGahan, Cormac G. M.
dc.contributor.authorSchellenberger, Pascale
dc.contributor.authorOgilvie, Lesley A.
dc.contributor.authorJones, Brian V.
dc.contributor.funderDunhill Medical Trusten
dc.contributor.funderMedical Research Councilen
dc.contributor.funderWellcome Trusten
dc.contributor.funderQueen Victoria Hospital NHS Foundation Trusten
dc.contributor.funderQueen Victoria Hospital Charitable Funden
dc.date.accessioned2019-09-17T13:48:31Z
dc.date.available2019-09-17T13:48:31Z
dc.date.issued2019-08-06
dc.description.abstractProteus mirabilis often complicates the care of catheterized patients through the formation of crystalline biofilms which block urine flow. Bacteriophage therapy has been highlighted as a promising approach to control this problem, but relatively few phages infecting P. mirabilis have been characterized. Here we characterize five phages capable of infecting P. mirabilis, including those shown to reduce biofilm formation, and provide insights regarding the wider ecological and evolutionary relationships of these phages. Transmission electron microscopy (TEM) imaging of phages vB_PmiP_RS1pmA, vB_PmiP_RS1pmB, vB_PmiP_RS3pmA, and vB_PmiP_RS8pmA showed that all share morphologies characteristic of the Podoviridae family. The genome sequences of vB_PmiP_RS1pmA, vB_PmiP_RS1pmB, and vB_PmiP_RS3pmA showed these are species of the same phage differing only by point mutations, and are closely related to vB_PmiP_RS8pmA. Podophages characterized in this study were also found to share similarity in genome architecture and composition to other previously described P. mirabilis podophages (PM16 and PM75). In contrast, vB_PimP_RS51pmB showed morphology characteristic of the Myoviridae family, with no notable similarity to other phage genomes examined. Ecogenomic profiling of all phages revealed no association with human urinary tract viromes, but sequences similar to vB_PimP_RS51pmB were found within human gut, and human oral microbiomes. Investigation of wider host-phage evolutionary relationships through tetranucleotide profiling of phage genomes and bacterial chromosomes, indicated vB_PimP_RS51pmB has a relatively recent association with Morganella morganii and other non-Proteus members of the Morganellaceae family. Subsequent host range assays confirmed vB_PimP_RS51pmB can infect M. morganii.en
dc.description.sponsorshipDunhill Medical Trust (R394/1114);en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid1783en
dc.identifier.citationAlves, D. R., Nzakizwanayo, J., Dedi, C., Olympiou, C., Hanin, A., Kot, W., Hansen, L., Lametsch, R., Gahan, C. G. M., Schellenberger, P., Ogilvie, L. A. and Jones, B. V. (2019) 'Genomic and Ecogenomic Characterization of Proteus mirabilis Bacteriophages', Frontiers in Microbiology, 10(1783). (14pp.) DOI: 10.3389/fmicb.2019.01783en
dc.identifier.doi10.3389/fmicb.2019.01783en
dc.identifier.eissn1664-302X
dc.identifier.endpage14en
dc.identifier.journaltitleFrontiers in Microbiologyen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/8544
dc.identifier.volume10en
dc.language.isoenen
dc.publisherFrontiers Mediaen
dc.relation.projectinfo:eu-repo/grantAgreement/RCUK/MRC/MR/N006496/1/GB/Development of an infection detecting wound dressing/en
dc.relation.projectinfo:eu-repo/grantAgreement/WT/Pathogen Biology and Disease Transmission/206854//Microneedles: Bypassing the Gastrointestinal Microbiota to Circumvent Antibiotic Resistance/en
dc.relation.urihttps://www.frontiersin.org/articles/10.3389/fmicb.2019.01783/full#fun1
dc.rights©2019 Alves, Nzakizwanayo, Dedi, Olympiou, Hanin, Kot, Hansen, Lametsch, Gahan, Schellenberger, Ogilvie and Jones. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectBacteriophageen
dc.subjectBiofilmen
dc.subjectEcogenomicsen
dc.subjectPhage therapyen
dc.subjectCathetersen
dc.titleGenomic and ecogenomic characterisation of Proteus mirabilis bacteriophageen
dc.typeArticle (peer-reviewed)en
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