RNA-Seq analysis of the guppy immune response against Gyrodactylus bullatarudis infection

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dc.contributor.authorKonczal, Mateusz
dc.contributor.authorEllison, Amy R.
dc.contributor.authorPhillips, Karl P.
dc.contributor.authorRadwan, Jacek
dc.contributor.authorMohammed, Ryan S.
dc.contributor.authorCable, Joanne
dc.contributor.authorChadzinska, Magdalena
dc.contributor.funderNarodowe Centrum Naukien
dc.contributor.funderPoznan RNA Research Centre, Polanden
dc.contributor.funderLlywodraeth Cymruen
dc.contributor.funderHigher Education Funding Council for Walesen
dc.contributor.funderBiotechnology and Biological Sciences Research Councilen
dc.contributor.funderScience Foundation Irelanden
dc.date.accessioned2020-09-17T08:52:16Z
dc.date.available2020-09-17T08:52:16Z
dc.date.issued2020-08-01
dc.date.updated2020-09-16T12:13:59Z
dc.description.abstractGyrodactylids are ubiquitous ectoparasites of teleost fish, but our understanding of the host immune response against them is fragmentary. Here, we used RNA-Seq to investigate genes involved in the primary response to infection with Gyrodactylus bullatarudis on the skin of guppies, Poecilia reticulata, an important evolutionary model, but also one of the most common fish in the global ornamental trade. Analysis of differentially expressed genes identified several immune-related categories, including IL-17 signalling pathway and Th17 cell differentiation, cytokine-cytokine receptor interaction, chemokine signalling pathway, NOD-like receptor signalling pathway, natural killer cell-mediated cytotoxicity and pathways involved in antigen recognition, processing and presentation. Components of both the innate and the adaptive immune responses play a role in response to gyrodactylid infection. Genes involved in IL-17/Th17 response were particularly enriched among differentially expressed genes, suggesting a significant role for this pathway in fish responses to ectoparasites. Our results revealed a sizable list of genes potentially involved in the teleost-gyrodactylid immune response.en
dc.description.sponsorshipNarodowe Centrum Nauki (Grant Numbers: UMO‐2013/10/M/NZ8/00253 , UMO‐2016/20/S/NZ8/00208); Poznan RNA Research Centre, Poland (Grant Number: 01/KNOW2/2014); Biotechnology and Biological Sciences Research Council (Future Leader Fellowship Grant Number: BB/R010609/1)en
dc.description.statusPeer revieweden
dc.description.versionAccepted Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleide12782en
dc.identifier.citationKonczal, M., Ellison, A. R., Phillips, K. P., Radwan, J., Mohammed, R. S., Cable, J. and Chadzinska, M. (2020) 'RNA-Seq analysis of the guppy immune response against Gyrodactylus bullatarudis infection', Parasite Immunology. doi: 10.1111/pim.12782en
dc.identifier.doi10.1111/pim.12782en
dc.identifier.eissn1365-3024
dc.identifier.issn0141-9838
dc.identifier.journaltitleParasite Immunologyen
dc.identifier.urihttps://hdl.handle.net/10468/10535
dc.language.isoenen
dc.publisherJohn Wiley & Sons, Ltd.en
dc.rights© 2020, John Wiley & Sons, Ltd. This is the peer reviewed version of the following article: Konczal, M., Ellison, A. R., Phillips, K. P., Radwan, J., Mohammed, R. S., Cable, J. and Chadzinska, M. (2020) 'RNA-Seq analysis of the guppy immune response against Gyrodactylus bullatarudis infection', Parasite Immunology, doi: 10.1111/pim.12782, which has been published in final form at https://doi.org/10.1111/pim.12782. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.en
dc.subjectEctoparasitesen
dc.subjectFishen
dc.subjectGuppyen
dc.subjectGyrodactylusen
dc.subjectImmunityen
dc.subjectRNA-Seqen
dc.subjectTh17 responseen
dc.subjectTranscriptomeen
dc.titleRNA-Seq analysis of the guppy immune response against Gyrodactylus bullatarudis infectionen
dc.typeArticle (peer-reviewed)en
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