Use of derivative diffuse reflectance spectroscopy in CAM assay combined with multivariate analysis as an approach to detect features of vulnerable plaques

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dc.contributor.authorLu, Huihuien
dc.contributor.authorO’Dowling, Claireen
dc.contributor.authorCaplice, Noelen
dc.contributor.authorBurke, Rayen
dc.contributor.authorAndersson-Engels, Stefanen
dc.contributor.editorContini, Davideen
dc.contributor.editorHoshi, Yokoen
dc.contributor.editorO'Sullivan, Thomas D.en
dc.contributor.funderScience Foundation Irelanden
dc.date.accessioned2023-10-12T14:53:15Z
dc.date.available2023-10-12T14:53:15Z
dc.date.issued2023-08-09en
dc.description.abstractIdentifying vulnerable plaques at an early stage is critical to reducing patient mortality associated with cardiovascular diseases. Diffuse reflectance spectroscopy (DRS) can directly measure of absorption and scattering properties of tissue and can detect oxy-haemoglobin inside the plaque associated with intraplaque haemorrhage, which is a feature of vulnerable plaques. This study assesses the potential of using a diffuse reflectance spectroscopy combined with a machine learning algorithm to identify oxygenated haemoglobin in a chick embryo chorioallantoic membrane (CAM) assay model. A total of 88 diffuse reflectance spectra measurements were collected from five 12 to 14-day-old embryos. The first and second derivative of the reflectance spectra were calculated, followed by the use of partial least square-linear discriminant analysis (PLS-LDA) to identify oxygenated haemoglobin in chick embryo vessels. The model achieved a sensitivity and specificity of 96% and 72%, respectively, in differentiating arteries from veins (oxy-haemoglobin) using reflectance data. The sensitivity and specificity were 92% and 88% using the first derivative of reflectance data, and 100% and 92% using the second derivative of reflectance data in the wavelength range of 500-600 nm. Initial results indicate that derivative reflectance combined with multivariate analysis has advantages for detecting tissue oxygenated haemoglobin in CAM assay model. This approach shows promise as a way to identify and study the features of vulnerable plaques.en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid126281Len
dc.identifier.citationLu, H., O’Dowling, C., Caplice, N. M., Burke, R. and Andersson-Engels, S. (2023) 'Use of derivative diffuse reflectance spectroscopy in CAM assay combined with multivariate analysis as an approach to detect features of vulnerable plaques', Proceedings of SPIE, 12628, Diffuse Optical Spectroscopy and Imaging IX, 126281L (5pp). doi: 10.1117/12.2670678en
dc.identifier.doi10.1117/12.2670678en
dc.identifier.eissn1996-756Xen
dc.identifier.endpage5en
dc.identifier.issn0277-786Xen
dc.identifier.journaltitleProceedings of SPIEen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/15113
dc.identifier.volume12628en
dc.language.isoenen
dc.publisherSociety of Photo-Optical Instrumentation Engineers (SPIE)en
dc.relation.ispartofEuropean Conferences on Biomeidcal Optics, 25-30 June 2023: Diffuse Optical Spectroscopy and Imaging IXen
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Research Professorship Programme/15/RP/2828/IE/Novel applications and techniques for in vivo optical imaging and spectroscopy/en
dc.rights© 2023, Society of Photo-Optical Instrumentation Engineers (SPIE). One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this publication for a fee or for commercial purposes, and modification of the contents of the publication are prohibited.en
dc.subjectVulnerable plaquesen
dc.subjectCardiovascular diseaseen
dc.subjectDiffuse reflectance spectroscopyen
dc.subjectReflectivityen
dc.subjectArteriesen
dc.subjectVeinsen
dc.subjectDiffuse reflectance spectroscopyen
dc.subjectContent addressable memoryen
dc.subjectTissues
dc.subjectData modelingen
dc.subjectOxygenationen
dc.subjectAtherosclerosisen
dc.subjectSpectroscopyen
dc.titleUse of derivative diffuse reflectance spectroscopy in CAM assay combined with multivariate analysis as an approach to detect features of vulnerable plaquesen
dc.typeConference itemen
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