Genetic and functional characterisation of the lactococcal P335 phage-host interactions
dc.contributor.author | Mahony, Jennifer | |
dc.contributor.author | Oliveira, Joana | |
dc.contributor.author | Collins, Barry | |
dc.contributor.author | Hanemaaijer, Laurens | |
dc.contributor.author | Lugli, Gabriele A. | |
dc.contributor.author | Neve, Horst | |
dc.contributor.author | Ventura, Marco | |
dc.contributor.author | Kouwen, Thijs R. | |
dc.contributor.author | Cambillau, Christian | |
dc.contributor.author | van Sinderen, Douwe | |
dc.contributor.funder | Science Foundation Ireland | en |
dc.contributor.funder | DSM Food Specialties, the Netherlands. | |
dc.date.accessioned | 2017-02-22T12:19:09Z | |
dc.date.available | 2017-02-22T12:19:09Z | |
dc.date.issued | 2017-02-10 | |
dc.date.updated | 2017-02-22T12:02:09Z | |
dc.description.abstract | Background: Despite continuous research efforts, bacterio(phages) infecting Lactococcus lactis starter strains persist as a major threat to dairy fermentations. The lactococcal P335 phages, which are currently classified into four sub-groups (I-IV), are the second most frequently isolated phage group in an industrial dairy context. Results: The current work describes the isolation and comparative genomic analysis of 17 novel P335 group phages. Detailed analysis of the genomic region of P335 phages encoding the so-called “baseplate”, which includes the receptor binding protein (RBP) was combined with a functional characterization of the RBP of sub-group III and IV phages. Additionally, calcium-dependence assays revealed a specific requirement for calcium by sub-group IV phages while host range analysis highlighted a higher number of strains with CWPS type A (11 of 39 strains) are infected by the P335 phages assessed in this study than those with a C (five strains), B (three of 39 strains) or unknown (one of 39 strains) CWPS type. Conclusions: These analyses revealed significant divergence among RBP sequences, apparently reflecting their unique interactions with the host and particularly for strains with a type A CWPS. The implications of the genomic architecture of lactococcal P335 phages on serving as a general model for Siphoviridae phages are discussed. | en |
dc.description.sponsorship | Science Foundation Ireland (SFI Technology Innovation Development Award (TIDA) (Ref. No. 14/TIDA/2287), Starting Investigator Research Grant (SIRG) (Ref. No. 15/SIRG/3430), SFI Principal Investigator award (Ref. No. 13/IA/1953)) DSM Food Specialties, the Netherlands. | en |
dc.description.status | Peer reviewed | en |
dc.description.version | Published Version | en |
dc.format.mimetype | application/pdf | en |
dc.identifier.citation | Mahony, J., Oliveira, J., Collins, B., Hanemaaijer, L., Lugli, G. A., Neve, H., Ventura, M., Kouwen, T. R., Cambillau, C. and van Sinderen, D. (2017) 'Genetic and functional characterisation of the lactococcal P335 phage-host interactions', BMC Genomics, 18(1), pp. 146. doi:10.1186/s12864-017-3537-5 | en |
dc.identifier.doi | 10.1186/s12864-017-3537-5 | |
dc.identifier.endpage | 146-16 | en |
dc.identifier.issn | 1471-2164 | |
dc.identifier.issued | 1 | en |
dc.identifier.journaltitle | BMC Genomics | en |
dc.identifier.startpage | 146-1 | en |
dc.identifier.uri | https://hdl.handle.net/10468/3666 | |
dc.identifier.volume | 18 | en |
dc.language.iso | en | en |
dc.publisher | BioMed Central | en |
dc.rights | © The Author(s). 2017 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. | en |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | en |
dc.subject | Bacteriophage | en |
dc.subject | Lactococcus lactis | en |
dc.subject | Dairy Fermentation | en |
dc.subject | Receptor-binding protein | en |
dc.title | Genetic and functional characterisation of the lactococcal P335 phage-host interactions | en |
dc.type | Article (peer-reviewed) | en |
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