Genetic and functional characterisation of the lactococcal P335 phage-host interactions

dc.contributor.authorMahony, Jennifer
dc.contributor.authorOliveira, Joana
dc.contributor.authorCollins, Barry
dc.contributor.authorHanemaaijer, Laurens
dc.contributor.authorLugli, Gabriele A.
dc.contributor.authorNeve, Horst
dc.contributor.authorVentura, Marco
dc.contributor.authorKouwen, Thijs R.
dc.contributor.authorCambillau, Christian
dc.contributor.authorvan Sinderen, Douwe
dc.contributor.funderScience Foundation Irelanden
dc.contributor.funderDSM Food Specialties, the Netherlands.
dc.date.accessioned2017-02-22T12:19:09Z
dc.date.available2017-02-22T12:19:09Z
dc.date.issued2017-02-10
dc.date.updated2017-02-22T12:02:09Z
dc.description.abstractBackground: Despite continuous research efforts, bacterio(phages) infecting Lactococcus lactis starter strains persist as a major threat to dairy fermentations. The lactococcal P335 phages, which are currently classified into four sub-groups (I-IV), are the second most frequently isolated phage group in an industrial dairy context. Results: The current work describes the isolation and comparative genomic analysis of 17 novel P335 group phages. Detailed analysis of the genomic region of P335 phages encoding the so-called “baseplate”, which includes the receptor binding protein (RBP) was combined with a functional characterization of the RBP of sub-group III and IV phages. Additionally, calcium-dependence assays revealed a specific requirement for calcium by sub-group IV phages while host range analysis highlighted a higher number of strains with CWPS type A (11 of 39 strains) are infected by the P335 phages assessed in this study than those with a C (five strains), B (three of 39 strains) or unknown (one of 39 strains) CWPS type. Conclusions: These analyses revealed significant divergence among RBP sequences, apparently reflecting their unique interactions with the host and particularly for strains with a type A CWPS. The implications of the genomic architecture of lactococcal P335 phages on serving as a general model for Siphoviridae phages are discussed.en
dc.description.sponsorshipScience Foundation Ireland (SFI Technology Innovation Development Award (TIDA) (Ref. No. 14/TIDA/2287), Starting Investigator Research Grant (SIRG) (Ref. No. 15/SIRG/3430), SFI Principal Investigator award (Ref. No. 13/IA/1953)) DSM Food Specialties, the Netherlands.en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationMahony, J., Oliveira, J., Collins, B., Hanemaaijer, L., Lugli, G. A., Neve, H., Ventura, M., Kouwen, T. R., Cambillau, C. and van Sinderen, D. (2017) 'Genetic and functional characterisation of the lactococcal P335 phage-host interactions', BMC Genomics, 18(1), pp. 146. doi:10.1186/s12864-017-3537-5en
dc.identifier.doi10.1186/s12864-017-3537-5
dc.identifier.endpage146-16en
dc.identifier.issn1471-2164
dc.identifier.issued1en
dc.identifier.journaltitleBMC Genomicsen
dc.identifier.startpage146-1en
dc.identifier.urihttps://hdl.handle.net/10468/3666
dc.identifier.volume18en
dc.language.isoenen
dc.publisherBioMed Centralen
dc.rights© The Author(s). 2017 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectBacteriophageen
dc.subjectLactococcus lactisen
dc.subjectDairy Fermentationen
dc.subjectReceptor-binding proteinen
dc.titleGenetic and functional characterisation of the lactococcal P335 phage-host interactionsen
dc.typeArticle (peer-reviewed)en
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