dc.contributor.author	Mahony, Jennifer
dc.contributor.author	Oliveira, Joana
dc.contributor.author	Collins, Barry
dc.contributor.author	Hanemaaijer, Laurens
dc.contributor.author	Lugli, Gabriele A.
dc.contributor.author	Neve, Horst
dc.contributor.author	Ventura, Marco
dc.contributor.author	Kouwen, Thijs R.
dc.contributor.author	Cambillau, Christian
dc.contributor.author	van Sinderen, Douwe
dc.contributor.funder	Science Foundation Ireland	en
dc.contributor.funder	DSM Food Specialties, the Netherlands.
dc.date.accessioned	2017-02-22T12:19:09Z
dc.date.available	2017-02-22T12:19:09Z
dc.date.issued	2017-02-10
dc.date.updated	2017-02-22T12:02:09Z
dc.description.abstract	Background: Despite continuous research efforts, bacterio(phages) infecting Lactococcus lactis starter strains persist as a major threat to dairy fermentations. The lactococcal P335 phages, which are currently classified into four sub-groups (I-IV), are the second most frequently isolated phage group in an industrial dairy context. Results: The current work describes the isolation and comparative genomic analysis of 17 novel P335 group phages. Detailed analysis of the genomic region of P335 phages encoding the so-called “baseplate”, which includes the receptor binding protein (RBP) was combined with a functional characterization of the RBP of sub-group III and IV phages. Additionally, calcium-dependence assays revealed a specific requirement for calcium by sub-group IV phages while host range analysis highlighted a higher number of strains with CWPS type A (11 of 39 strains) are infected by the P335 phages assessed in this study than those with a C (five strains), B (three of 39 strains) or unknown (one of 39 strains) CWPS type. Conclusions: These analyses revealed significant divergence among RBP sequences, apparently reflecting their unique interactions with the host and particularly for strains with a type A CWPS. The implications of the genomic architecture of lactococcal P335 phages on serving as a general model for Siphoviridae phages are discussed.	en
dc.description.sponsorship	Science Foundation Ireland (SFI Technology Innovation Development Award (TIDA) (Ref. No. 14/TIDA/2287), Starting Investigator Research Grant (SIRG) (Ref. No. 15/SIRG/3430), SFI Principal Investigator award (Ref. No. 13/IA/1953)) DSM Food Specialties, the Netherlands.	en
dc.description.status	Peer reviewed	en
dc.description.version	Published Version	en
dc.format.mimetype	application/pdf	en
dc.identifier.citation	Mahony, J., Oliveira, J., Collins, B., Hanemaaijer, L., Lugli, G. A., Neve, H., Ventura, M., Kouwen, T. R., Cambillau, C. and van Sinderen, D. (2017) 'Genetic and functional characterisation of the lactococcal P335 phage-host interactions', BMC Genomics, 18(1), pp. 146. doi:10.1186/s12864-017-3537-5	en
dc.identifier.doi	10.1186/s12864-017-3537-5
dc.identifier.endpage	146-16	en
dc.identifier.issn	1471-2164
dc.identifier.issued	1	en
dc.identifier.journaltitle	BMC Genomics	en
dc.identifier.startpage	146-1	en
dc.identifier.uri	https://hdl.handle.net/10468/3666
dc.identifier.volume	18	en
dc.language.iso	en	en
dc.publisher	BioMed Central	en
dc.rights	© The Author(s). 2017 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.	en
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/	en
dc.subject	Bacteriophage	en
dc.subject	Lactococcus lactis	en
dc.subject	Dairy Fermentation	en
dc.subject	Receptor-binding protein	en
dc.title	Genetic and functional characterisation of the lactococcal P335 phage-host interactions	en
dc.type	Article (peer-reviewed)	en

Genetic and functional characterisation of the lactococcal P335 phage-host interactions

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