Enhancing microbial superoxide generation and conversion to hydroxyl radicals for enhanced bioremediation using iron-binding ligands

Simple item page
dc.check.date2026-02-01en
dc.check.infoAccess to this article is restricted until 24 months after publication by request of the publisheren
dc.contributor.authorWang, Yuhanen
dc.contributor.authorNing, Xueen
dc.contributor.authorLiang, Jinsongen
dc.contributor.authorWang, Aijieen
dc.contributor.authorQu, Jiuhuien
dc.contributor.funderNational Natural Science Foundation of Chinaen
dc.contributor.funderShenzhen Science and Technology Innovation Programen
dc.date.accessioned2024-02-29T12:36:36Z
dc.date.available2024-02-29T12:36:36Z
dc.date.issued2024-02-01en
dc.description.abstractHarnessing bacteria for superoxide production in bioremediation holds immense promise, yet its practical application is hindered by slow production rates and the relatively weak redox potential of superoxide. This study delves into a cost-effective approach to amplify superoxide production using an Arthrobacter strain, a prevalent soil bacterial genus. Our research reveals that introducing a carbon source along with specific iron-binding ligands, including deferoxamine (DFO), diethylenetriamine pentaacetate (DTPA), citrate, and oxalate, robustly augments microbial superoxide generation. Moreover, our findings suggest that these iron-binding ligands play a pivotal role in converting superoxide into hydroxyl radicals by modulating the electron transfer rate between Fe(III)/Fe(II) and superoxide. Remarkably, among the tested ligands, only DTPA emerges as a potent promoter of this conversion process when complexed with Fe(III). We identify an optimal Fe(III) to DTPA ratio of approximately 1:1 for enhancing hydroxyl radical production within the Arthrobacter culture. This research underscores the efficacy of simultaneously introducing carbon sources and DTPA in facilitating superoxide production and its subsequent conversion to hydroxyl radicals, significantly elevating bioremediation performance. Furthermore, our study reveals that DTPA augments superoxide production in cultures of diverse soils, with various soil microorganisms beyond Arthrobacter identified as contributors to superoxide generation. This emphasizes the universal applicability of DTPA across multiple bacterial genera. In conclusion, our study introduces a promising methodology for enhancing microbial superoxide production and its conversion into hydroxyl radicals. These findings hold substantial implications for the deployment of microbial reactive oxygen species in bioremediation, offering innovative solutions for addressing environmental contamination challenges.en
dc.description.sponsorshipNational Natural Science Foundation of China (Nos. 52170156; 52250056; 52293443); Shenzhen Science and Technology Innovation Program (No. KQTD20190929172630447)en
dc.description.statusPeer revieweden
dc.description.versionAccepted Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationWang, Y., Ning, X., Liang, J., Wang, A. and Qu, J. (2024) 'Enhancing microbial superoxide generation and conversion to hydroxyl radicals for enhanced bioremediation using iron-binding ligands', Journal of Environmental Sciences, 147, pp. 597-606. https://doi.org/10.1016/j.jes.2023.11.023en
dc.identifier.doihttps://doi.org/10.1016/j.jes.2023.11.023en
dc.identifier.endpage606en
dc.identifier.issn1001-0742en
dc.identifier.journaltitleJournal of Environmental Sciencesen
dc.identifier.startpage597en
dc.identifier.urihttps://hdl.handle.net/10468/15610
dc.identifier.volume147en
dc.language.isoenen
dc.publisherElsevier B.V.en
dc.rights© 2024 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V. All rights reserved. This manuscript version is made available under the CC BY-NC-ND 4.0 license.en
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectSuperoxideen
dc.subjectHydroxyl radicalen
dc.subjectIron-binding liganden
dc.subjectBioremediationen
dc.subjectDTPAen
dc.titleEnhancing microbial superoxide generation and conversion to hydroxyl radicals for enhanced bioremediation using iron-binding ligandsen
dc.typeArticle (peer-reviewed)en
oaire.citation.volume147en
Files
Original bundle
Now showing 1 - 2 of 2
Thumbnail Image
Name:
manuscript.pdf
Size:
601 KB
Format:
Adobe Portable Document Format
Description:
Accepted Version
Thumbnail Image
Name:
Manuscript.docx
Size:
1.96 MB
Format:
Microsoft Word XML
Description:
Authors' original accepted version
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
2.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Centre for Marine and Renewable Energy (MaREI) - Journal Articles