Nisin E is a novel nisin variant produced by multiple Streptococcus equinus strains

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dc.contributor.authorSugrue, Ivan
dc.contributor.authorHill, Daragh
dc.contributor.authorO'Connor, Paula M.
dc.contributor.authorDay, Li
dc.contributor.authorStanton, Catherine
dc.contributor.authorHill, Colin
dc.contributor.authorRoss, R. Paul
dc.contributor.funderTeagascen
dc.contributor.funderJPI Food Processing for Health Longlife Projecten
dc.contributor.funderScience Foundation Irelanden
dc.date.accessioned2023-03-15T11:53:52Z
dc.date.available2023-03-15T11:53:52Z
dc.date.issued2023-02
dc.date.updated2023-03-10T14:15:02Z
dc.description.abstractNisin A, the prototypical lantibiotic, is an antimicrobial peptide currently utilised as a food preservative, with potential for therapeutic applications. Here, we describe nisin E, a novel nisin variant produced by two Streptococcus equinus strains, APC4007 and APC4008, isolated from sheep milk. Shotgun whole genome sequencing and analysis revealed biosynthetic gene clusters similar to nisin U, with a unique rearrangement of the core peptide encoding gene within the cluster. The 3100.8 Da peptide by MALDI-TOF mass spectrometry, is 75% identical to nisin A, with 10 differences, including 2 deletions: Ser29 and Ile30, and 8 substitutions: Ile4Lys, Gly18Thr, Asn20Pro, Met21Ile, His27Gly, Val32Phe, Ser33Gly, and Lys34Asn. Nisin E producing strains inhibited species of Lactobacillus, Bacillus, and Clostridiodes and were immune to nisin U. Sequence alignment identified putative promoter sequences across the nisin producer genera, allowing for the prediction of genes in Streptococcus to be potentially regulated by nisin. S. equinus pangenome BLAST analyses detected 6 nisin E operons across 44 publicly available genomes. An additional 20 genomes contained a subset of nisin E transport/immunity and regulatory genes (nseFEGRK), without adjacent peptide production genes. These genes suggest that nisin E response mechanisms, distinct from the canonical nisin immunity and resistance operons, are widespread across the S. equinus species. The discovery of this new nisin variant and its immunity determinants in S. equinus suggests a central role for nisin in the competitive nature of the species.en
dc.description.sponsorshipTeagasc (Walsh Scholarship)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid427en
dc.identifier.citationSugrue, I., Hill, D., O’Connor, P.M., Day, L., Stanton, C., Hill, C. and Ross, R.P. (2023) ‘Nisin E is a novel nisin variant produced by multiple streptococcus equinus strains’, Microorganisms, 11(2), 427 (19pp). doi: 10.3390/microorganisms11020427en
dc.identifier.doi10.3390/microorganisms11020427en
dc.identifier.endpage19en
dc.identifier.issn2076-2607
dc.identifier.issued2en
dc.identifier.journaltitleMicroorganismsen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/14308
dc.identifier.volume11en
dc.language.isoenen
dc.publisherMDPIen
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2273/IE/Alimentary Pharmabiotic Centre (APC) - Interfacing Food & Medicine/en
dc.rights© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/)en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectNisinen
dc.subjectStreptococcus equinusen
dc.subjectBacteriocinen
dc.subjectLantibioticen
dc.subjectAntimicrobial peptideen
dc.subjectBiotherapeuticen
dc.titleNisin E is a novel nisin variant produced by multiple Streptococcus equinus strainsen
dc.typeArticle (peer-reviewed)en
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