Orally administered CLA ameliorates DSS-induced colitis in mice via intestinal barrier improvement, oxidative stress reduction, inflammatory cytokine and gut microbiota modulation

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dc.contributor.authorChen, Yang
dc.contributor.authorYang, Bo
dc.contributor.authorRoss, R. Paul
dc.contributor.authorJin, Yan
dc.contributor.authorStanton, Catherine
dc.contributor.authorZhao, Jianxin
dc.contributor.authorZhang, Hao
dc.contributor.authorChen, Wei
dc.contributor.funderNational Natural Science Foundation of Chinaen
dc.contributor.funderFundamental Research Funds for the Central Universitiesen
dc.contributor.funderNational First-Class Discipline Program of Food Science and Technology, Chinaen
dc.contributor.funderPostgraduate Research and Practice Innovation Program of Jiangsu Province, Chinaen
dc.contributor.funderWuxi Young Talent Foundation, Chinaen
dc.contributor.funderJiangsu Province Collaborative Innovation Center for Food Safety and Quality Control, Chinaen
dc.date.accessioned2020-01-14T11:00:03Z
dc.date.available2020-01-14T11:00:03Z
dc.date.issued2019-11-06
dc.date.updated2019-11-14T09:38:03Z
dc.description.abstractDietary supplementation with CLA has been reported to alleviate the effect of colitis in mice, but the mechanisms involved need further exploration. The study aimed to investigate how orally administered CLA alleviates DSS-induced colitis in mice. CLA was administered at five different doses: 40 mg/d, 20 mg/d, 10 mg/d, 5 mg/d and 2.5 mg/d. Doses of CLA at 10 mg/d and higher alleviated colitis symptoms and reduced inflammation induced by DSS, in which 40 mg/d, 20 mg/d and 10 mg/d CLA significantly increased the concentration of MUC2 and goblet cells, but neither 5 mg/d CLA nor 2.5 mg/d CLA had any effects. Meanwhile, 40 mg/d CLA and 20 mg/d CLA treatments significantly up-regulated the concentration of tight junction proteins (ZO-1, occludin and claudin-3) and ameliorated epithelial apoptosis caused by DSS. Moreover, oxidative stress-related enzymes (SOD, GSH-PX, CAT) and inflammatory cytokines (TNF-a, IL-10, IL-6) were modulated by 40 mg/d CLA and 20 mg/d CLA. Furthermore, 40 mg/d CLA rebalanced the gut microbiota damaged by DSS, including reducing Bacteroides and increasing Bifidobacterium and Odoribacter. In conclusion, CLA supplementation alleviated DSS-induced colitis in a dose-dependent manner by modulating inflammatory cytokines and oxidation stress, maintaining the mucosal barrier and reverting microbiota changes.en
dc.description.sponsorshipNational Natural Science Foundation of China (Nos. 31801521; 31722041); Fundamental Research Funds for the Central Universities (No. JUSRP51702A); National First-Class Discipline Program of Food Science and Technology, China (JUFSTR20180102); Postgraduate Research and Practice Innovation Program of Jiangsu Province, China (KYCX19_1829); Wuxi Young Talent Foundation, China (QNRC075)en
dc.description.statusPeer revieweden
dc.description.versionAccepted Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationChen, Y., Yang, B., Ross, R. P., Jin, Y., Stanton, C., Zhao, J., Zhang, H. and Chen, W. (2019) 'Orally administered CLA ameliorates DSS-induced colitis in mice via intestinal barrier improvement, oxidative stress reduction, inflammatory cytokine and gut microbiota modulation', Journal of Agricultural and Food Chemistry, 67(48), pp. 13282-13298. doi: 10.1021/acs.jafc.9b05744en
dc.identifier.doi10.1021/acs.jafc.9b05744en
dc.identifier.eissn1520-5118
dc.identifier.endpage13298en
dc.identifier.issn0021-8561
dc.identifier.issued48en
dc.identifier.journaltitleJournal of Agricultural and Food Chemistryen
dc.identifier.startpage13282en
dc.identifier.urihttps://hdl.handle.net/10468/9500
dc.identifier.volume67en
dc.language.isoenen
dc.publisherACS Publicationsen
dc.rights© 2019, American Chemical Society. This document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of Agricultural and Food Chemistry, © American Chemical Society, after peer review and technical editing by the publisher. To access the final edited and published work see https://pubs.acs.org/doi/abs/10.1021/acs.jafc.9b05744en
dc.subjectConjugated linoleic aciden
dc.subjectColitisen
dc.subjectIntestinal barrier functionen
dc.subjectOxidative stressen
dc.subjectGut microbiotaen
dc.titleOrally administered CLA ameliorates DSS-induced colitis in mice via intestinal barrier improvement, oxidative stress reduction, inflammatory cytokine and gut microbiota modulationen
dc.typeArticle (peer-reviewed)en
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