Comparative genomics and functional analysis of the 936 group of lactococcal Siphoviridae phages

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dc.contributor.authorMurphy, James
dc.contributor.authorBottacini, Francesca
dc.contributor.authorMahony, Jennifer
dc.contributor.authorKelleher, Philip
dc.contributor.authorNeve, Horst
dc.contributor.authorZomer, Aldert
dc.contributor.authorNauta, Arjen
dc.contributor.authorvan Sinderen, Douwe
dc.contributor.funderScience Foundation Irelanden
dc.contributor.funderIrish Research Councilen
dc.date.accessioned2017-02-23T12:33:05Z
dc.date.available2017-02-23T12:33:05Z
dc.date.issued2016-02-19
dc.date.updated2017-02-23T12:24:55Z
dc.description.abstractGenome sequencing and comparative analysis of bacteriophage collections has greatly enhanced our understanding regarding their prevalence, phage-host interactions as well as the overall biodiversity of their genomes. This knowledge is very relevant to phages infecting Lactococcus lactis, since they constitute a significant risk factor for dairy fermentations. Of the eighty four lactococcal phage genomes currently available, fifty five belong to the so-called 936 group, the most prevalent of the ten currently recognized lactococcal phage groups. Here, we report the genetic characteristics of a new collection of 936 group phages. By combining these genomes to those sequenced previously we determined the core and variable elements of the 936 genome. Genomic variation occurs across the 936 phage genome, such as genetic elements that (i) lead to a +1 translational frameshift resulting in the formation of additional structures on the phage tail, (ii) specify a double neck passage structure, and (iii) encode packaging module-associated methylases. Hierarchical clustering of the gene complement of the 936 group phages and nucleotide alignments allowed grouping of the ninety 936 group phages into distinct clusters, which in general appear to correspond with their geographical origin.en
dc.description.sponsorshipScience Foundation Ireland (SFI Investigator award (Ref. No.13/IA/1953), SFI Technology Innovation Development Award (TIDA) (Ref. No. 14/TIDA/2287)); Irish Research Council (Enterprise Partnership Scheme postgraduate scholarship)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationMurphy, J., Bottacini, F., Mahony, J., Kelleher, P., Neve, H., Zomer, A., Nauta, A. and van Sinderen, D. (2016) 'Comparative genomics and functional analysis of the 936 group of lactococcal Siphoviridae phages', Scientific Reports, 6, pp. 21345. doi:10.1038/srep21345en
dc.identifier.doi10.1038/srep21345
dc.identifier.endpage21345-13en
dc.identifier.issn2045-2322
dc.identifier.journaltitleScientific Reportsen
dc.identifier.startpage21345-1en
dc.identifier.urihttps://hdl.handle.net/10468/3676
dc.identifier.volume6en
dc.language.isoenen
dc.publisherNature Publishing Groupen
dc.rights© 2016, The Authors. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectPhage biologyen
dc.subjectSequence annotationen
dc.subjectInfection mechanismen
dc.subjectLytic bacteriophageen
dc.subjectGene expressionen
dc.subjectReceptor binding proteinen
dc.titleComparative genomics and functional analysis of the 936 group of lactococcal Siphoviridae phagesen
dc.typeArticle (peer-reviewed)en
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