Diverse and abundant secondary metabolism biosynthetic gene clusters in the genomes of marine sponge derived Streptomyces spp. Isolates

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dc.contributor.authorJackson, Stephen A.
dc.contributor.authorCrossman, Lisa
dc.contributor.authorAlmeida, Eduardo L.
dc.contributor.authorMargassery, Lekha Menon
dc.contributor.authorKennedy, Jonathan
dc.contributor.authorDobson, Alan D. W.
dc.contributor.funderEarth and Life Systems Alliance
dc.contributor.funderSeventh Framework Programme
dc.date.accessioned2018-05-02T10:16:24Z
dc.date.available2018-05-02T10:16:24Z
dc.date.issued2018
dc.description.abstractThe genus Streptomyces produces secondary metabolic compounds that are rich in biological activity. Many of these compounds are genetically encoded by large secondary metabolism biosynthetic gene clusters (smBGCs) such as polyketide synthases (PKS) and non-ribosomal peptide synthetases (NRPS) which are modular and can be highly repetitive. Due to the repeats, these gene clusters can be difficult to resolve using short read next generation datasets and are often quite poorly predicted using standard approaches. We have sequenced the genomes of 13 Streptomyces spp. strains isolated from shallow water and deep-sea sponges that display antimicrobial activities against a number of clinically relevant bacterial and yeast species. Draft genomes have been assembled and smBGCs have been identified using the antiSMASH (antibiotics and Secondary Metabolite Analysis Shell) web platform. We have compared the smBGCs amongst strains in the search for novel sequences conferring the potential to produce novel bioactive secondary metabolites. The strains in this study recruit to four distinct clades within the genus Streptomyces. The marine strains host abundant smBGCs which encode polyketides, NRPS, siderophores, bacteriocins and lantipeptides. The deep-sea strains appear to be enriched with gene clusters encoding NRPS. Marine adaptations are evident in the sponge-derived strains which are enriched for genes involved in the biosynthesis and transport of compatible solutes and for heat-shock proteins. Streptomyces spp. from marine environments are a promising source of novel bioactive secondary metabolites as the abundance and diversity of smBGCs show high degrees of novelty. Sponge derived Streptomyces spp. isolates appear to display genomic adaptations to marine living when compared to terrestrial strains.en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid67
dc.identifier.citationJackson, S., Crossman, L., Almeida, E., Margassery, L., Kennedy, J. and Dobson, A. (2018) 'Diverse and abundant secondary metabolism biosynthetic gene clusters in the genomes of marine sponge derived Streptomyces spp. Isolates', Marine Drugs, 16(2), 67 (18pp). doi: 10.3390/md16020067en
dc.identifier.doi10.3390/md16020067
dc.identifier.issn1660-3397
dc.identifier.issued2
dc.identifier.journaltitleMarine Drugsen
dc.identifier.urihttps://hdl.handle.net/10468/5938
dc.identifier.volume16
dc.language.isoenen
dc.publisherMDPI AGen
dc.relation.projectinfo:eu-repo/grantAgreement/EC/FP7::SP1::KBBE/312184/EU/Increasing Value and Flow in the Marine Biodiscovery Pipeline/PHARMASEA
dc.relation.urihttp://www.mdpi.com/1660-3397/16/2/67
dc.rights© 2018, the Authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectMarine streptomycesen
dc.subjectBiosynthetic gene clustersen
dc.subjectSecondary metabolitesen
dc.titleDiverse and abundant secondary metabolism biosynthetic gene clusters in the genomes of marine sponge derived Streptomyces spp. Isolatesen
dc.typeArticle (peer-reviewed)en
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