Reconstruction of the bifidobacterial pan-secretome reveals the network of extracellular interactions between bifidobacteria and the infant gut

dc.contributor.authorLugli, Gabriele A.
dc.contributor.authorMancino, Walter
dc.contributor.authorMilani, Christian
dc.contributor.authorDuranti, Sabrina
dc.contributor.authorTurroni, Francesca
dc.contributor.authorvan Sinderen, Douwe
dc.contributor.authorVentura, Marco
dc.contributor.funderScience Foundation Irelanden
dc.contributor.funderEuropean Commissionen
dc.contributor.funderFondazione Cariparmaen
dc.date.accessioned2019-08-26T13:52:10Z
dc.date.available2019-08-26T13:52:10Z
dc.date.issued2018-06-08
dc.date.updated2019-08-26T13:38:12Z
dc.description.abstractThe repertoire of secreted proteins decoded by a microorganism represents proteins released from or associated with the cell surface. In gut commensals, such as bifidobacteria, these proteins are perceived to be functionally relevant, as they regulate the interaction with the gut environment. In the current study, we screened the predicted proteome of over 300 bifidobacterial strains among the currently recognized bifidobacterial species to generate a comprehensive database encompassing bifidobacterial extracellular proteins. A glycobiome analysis of this predicted bifidobacterial secretome revealed that a correlation exists between particular bifidobacterial species and their capability to hydrolyze human milk oligosaccharides (HMOs) and intestinal glycoconjugates, such as mucin. Furthermore, an exploration of metatranscriptomic data sets of the infant gut microbiota allowed the evaluation of the expression of bifidobacterial genes encoding extracellular proteins, represented by ABC transporter substrate-binding proteins and glycoside hydrolases enzymes involved in the degradation of human milk oligosaccharides and mucin. Overall, this study provides insights into how bifidobacteria interact with their natural yet highly complex environment, the infant gut. IMPORTANCE: The ecological success of bifidobacteria relies on the activity of extracellular proteins that are involved in the metabolism of nutrients and the interaction with the environment. To date, information on secreted proteins encoded by bifidobacteria is incomplete and just related to few species. In this study, we reconstructed the bifidobacterial pan-secretome, revealing extracellular proteins that modulate the interaction of bifidobacteria with their natural environment. Furthermore, a survey of the secretion systems between bifidobacterial genomes allowed the identification of a conserved Sec-dependent secretion machinery in all the analyzed genomes and the Tat protein translocation system in the chromosomes of 23 strains belonging to Bifidobacterium longum subsp. longum and Bifidobacterium aesculapii.en
dc.description.sponsorshipEuropean Commission (EU Joint Programming Initiative “A Healthy Diet for a Healthy Life” (JPI HDHL; http://www.healthydietforhealthylife.eu/), (in conjunction with Science Fondation Ireland [SFI], grant 15/JP-HDHL/3280)); Fondazione Cariparma (under the TeachInParma Project);en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleide00796-18en
dc.identifier.citationLugli, G. A., Mancino, W., Milani, C., Duranti, S., Turroni, F., van Sinderen, D. and Ventura, M. (2018) 'Reconstruction of the Bifidobacterial Pan-Secretome Reveals the Network of Extracellular Interactions between Bifidobacteria and the Infant Gut', Applied and Environmental Microbiology, 84(16), e00796-18 (12 pp). doi: 10.1128/aem.00796-18en
dc.identifier.doi10.1128/aem.00796-18en
dc.identifier.endpage12en
dc.identifier.issn0099-2240
dc.identifier.issued16en
dc.identifier.journaltitleApplied and Environmental Microbiologyen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/8391
dc.identifier.volume84en
dc.language.isoenen
dc.publisherAmerican Society for Microbiologyen
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2273/IE/Alimentary Pharmabiotic Centre (APC) - Interfacing Food & Medicine/en
dc.relation.urihttps://aem.asm.org/content/aem/84/16/e00796-18.full.pdf
dc.rights© 2018 American Society for Microbiology. All Rights Reserved.en
dc.subjectBifidobacteriumen
dc.subjectBifidobacteriaen
dc.subjectGenomicsen
dc.subjectMetagenomicsen
dc.subjectSecretomeen
dc.titleReconstruction of the bifidobacterial pan-secretome reveals the network of extracellular interactions between bifidobacteria and the infant guten
dc.typeArticle (peer-reviewed)en
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