Use of enhanced nisin derivatives in combination with food-grade oils or citric acid to control Cronobacter sakazakii and Escherichia coli O157:H7

Simple item page
dc.contributor.authorCampion, Alicia M.
dc.contributor.authorMorrissey, Ruth M.
dc.contributor.authorField, Des
dc.contributor.authorCotter, Paul D.
dc.contributor.authorHill, Colin
dc.contributor.authorRoss, R. Paul
dc.contributor.funderScience Foundation Irelanden
dc.contributor.funderHigher Education Authorityen
dc.date.accessioned2017-05-18T08:31:22Z
dc.date.available2017-05-18T08:31:22Z
dc.date.issued2017-02-07
dc.date.updated2017-05-17T15:50:59Z
dc.description.abstractCronobacter sakazakii and Escherichia coli O157:H7 are well known food-borne pathogens that can cause severe disease. The identification of new alternatives to heating to control these pathogens in foods, while reducing the impact on organoleptic properties and nutritional value, is highly desirable. In this study, nisin and its bioengineered variants, nisin V and nisin S29A, are used alone, or in combination with plant essential oils (thymol, carvacrol and trans-cinnamaldehyde) or citric acid, with a view to controlling C. sakazakii and E. coli O157:H7 in laboratory-based assays and model food systems. The use of nisin variants (30 μM) with low concentrations of thymol (0.015%), carvacrol (0.03%) and trans-cinnamaldehyde (0.035%) resulted in extended lag phases of growth compared to those for corresponding nisin A-essential oil combinations. Furthermore, nisin variants (60 μM) used in combination with carvacrol (0.03%) significantly reduced viable counts of E. coli O157:H7 (3-log) and C. sakazakii (4-log) compared to nisin A-carvacrol treatment. Importantly, this increased effectiveness translated into food. More specifically, sub-inhibitory concentrations of nisin variants and carvacrol caused complete inactivation of E. coli O157:H7 in apple juice within 3 h at room temperature compared to that of the equivalent nisin A combination. Furthermore, combinations of commercial Nisaplin and the food additive citric acid reduced C. sakazakii numbers markedly in infant formula within the same 3 h period. These results highlight the potential benefits of combining nisin and variants thereof with carvacrol and/or citric acid for the inhibition of Gram negative food-borne pathogens.en
dc.description.sponsorshipHigher Education Authority (PhD Programme in Molecular Cell Biology funded by the Programme for Research in Third-Level Institutions (PRTLI)); Irish Government (National Development Plan); Science Foundation Ireland (SFI Investigator awards (10/IN.1/B3027) and (06/IN.1/B98))en
dc.description.statusPeer revieweden
dc.description.versionAccepted Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationCampion, A., Morrissey, R., Field, D., Cotter, P. D., Hill, C. and Ross, R. P. (2017) 'Use of enhanced nisin derivatives in combination with food-grade oils or citric acid to control Cronobacter sakazakii and Escherichia coli O157:H7', Food Microbiology, 65, pp. 254-263. doi:10.1016/j.fm.2017.01.020en
dc.identifier.doi10.1016/j.fm.2017.01.020
dc.identifier.endpage263en
dc.identifier.issn0740-0020
dc.identifier.journaltitleFood Microbiologyen
dc.identifier.startpage254en
dc.identifier.urihttps://hdl.handle.net/10468/3980
dc.identifier.volume65en
dc.language.isoenen
dc.publisherElsevieren
dc.rights© 2017 Elsevier Ltd. This manuscript version is made available under the CC-BY-NC-ND 4.0 licenseen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectNisinen
dc.subjectEssential oilsen
dc.subjectApple juiceen
dc.subjectInfant formula milken
dc.subjectC. sakazakiien
dc.subjectE. coli O157:H7en
dc.titleUse of enhanced nisin derivatives in combination with food-grade oils or citric acid to control Cronobacter sakazakii and Escherichia coli O157:H7en
dc.typeArticle (peer-reviewed)en
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
2515.pdf
Size:
8.08 MB
Format:
Adobe Portable Document Format
Description:
Accepted version
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
2.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Microbiology - Journal Articles
APC Microbiome Ireland - Journal Articles