Role of the intertidal predatory shore crab Carcinus maenas in transmission dynamics of ostreid herpesvirus-1 microvariant

Bookelaar, Babette E.; O'Reilly, A. J.; Lynch, Sharon A.; Culloty, Sarah C.

Role of the intertidal predatory shore crab Carcinus maenas in transmission dynamics of ostreid herpesvirus-1 microvariant

dc.contributor.author	Bookelaar, Babette E.
dc.contributor.author	O'Reilly, A. J.
dc.contributor.author	Lynch, Sharon A.
dc.contributor.author	Culloty, Sarah C.
dc.contributor.funder	Department of Agriculture, Food and the Marine	en
dc.date.accessioned	2018-11-13T13:36:01Z
dc.date.available	2018-11-13T13:36:01Z
dc.date.issued	2018-09-27
dc.date.updated	2018-11-13T13:25:24Z
dc.description.abstract	Ostreid herpesvirus-1 microVar (OsHV-1 µVar) has been responsible for significant mortalities globally in the Pacific oyster Crassostrea gigas. While the impact of this virus on the Pacific oyster has been significant, this pathogen may have wider ecosystem consequences. It has not been definitively determined how the virus is sustaining itself in the marine environment and whether other species are susceptible. The shore crab Carcinus maenas is a mobile predator and scavenger of C. gigas, commonly found at Pacific oyster culture sites. The aim of this study was to investigate the role of the crab in viral maintenance and transmission to the Pacific oyster. A field trial took place over 1 summer at different shore heights at 2 Irish Pacific oyster culture sites that are endemic for OsHV-1 µVar. Infection of OsHV-1 µVar in tissues of C. maenas at both shore heights of both sites was detected by polymerase chain reaction (PCR), quantitative PCR (qPCR), in situ hybridization and direct Sanger sequencing. In addition, a laboratory trial demonstrated that transmission of the virus could occur to naïve C. gigas within 4 d, from C. maenas previously exposed to the virus in the wild. These findings provide some insight into the possibility that the virus can be transmitted through marine food webs. The results also suggest viral plasticity in the hosts required by the virus and potential impacts on a range of crustacean species with wider ecosystem impacts if transmission to other species occurs.	en
dc.description.sponsorship	Department of Agriculture, Food and the Marine (REPOSUS Project ‘Reducing the impact of pathogens and disease in the Irish oyster industry to support the sustainability and growth of the sector’ 14 SF 820)	en
dc.description.status	Peer reviewed	en
dc.description.version	Published Version	en
dc.format.mimetype	application/pdf	en
dc.identifier.citation	Bookelaar, B. E., O'Reilly, A. J., Lynch, S. A. and Culloty, S. C. (2018) 'Role of the intertidal predatory shore crab Carcinus maenas in transmission dynamics of ostreid herpesvirus-1 microvariant', Diseases of Aquatic Organisms, 130(3), pp. 221-233. doi:10.3354/dao03264	en
dc.identifier.doi	10.3354/dao03264
dc.identifier.endpage	233	en
dc.identifier.issn	0177-5103
dc.identifier.issn	1616-1580
dc.identifier.issued	3	en
dc.identifier.journaltitle	Diseases of Aquatic Organisms	en
dc.identifier.startpage	221	en
dc.identifier.uri	https://hdl.handle.net/10468/7107
dc.identifier.volume	130	en
dc.language.iso	en	en
dc.publisher	Inter-Research	en
dc.relation.uri	https://www.int-res.com/abstracts/dao/v130/n3/p221-233/
dc.rights	© 2018, Inter-Research. All rights reserved.	en
dc.subject	Crassostrea gigas	en
dc.subject	Carcinus maenas	en
dc.subject	Scavenger	en
dc.subject	Ostreid herpesvirus-1 microvar	en
dc.subject	Pathogen-host-environment interplay	en
dc.subject	Predator-prey	en
dc.title	Role of the intertidal predatory shore crab Carcinus maenas in transmission dynamics of ostreid herpesvirus-1 microvariant	en
dc.type	Article (peer-reviewed)	en