Atypical Listeria innocua strains possess an intact LIPI-3

dc.contributor.authorClayton, Evelyn M.
dc.contributor.authorDaly, Karen M.
dc.contributor.authorGuinane, Caitriona M.
dc.contributor.authorHill, Colin
dc.contributor.authorCotter, Paul D.
dc.contributor.authorRoss, R. Paul
dc.contributor.funderEnterprise Irelanden
dc.contributor.funderScience Foundation Irelanden
dc.contributor.funderEuropean Commission
dc.date.accessioned2016-01-29T13:24:03Z
dc.date.available2016-01-29T13:24:03Z
dc.date.issued2014-03-08
dc.description.abstractBackground: Listeria monocytogenes is a food-borne pathogen which is the causative agent of listeriosis and can be divided into three evolutionary lineages I, II and III. While all strains possess the well established virulence factors associated with the Listeria pathogenicity island I (LIPI-1), lineage I strains also possess an additional pathogenicity island designated LIPI-3 which encodes listeriolysin S (LLS), a post-translationally modified cytolytic peptide. Up until now, this pathogenicity island has been identified exclusively in a subset of lineage I isolates of the pathogen Listeria monocytogenes. Results: In total 64 L. innocua strains were screened for the presence of LIPI-3. Here we report the identification of an intact LIPI-3 in 11 isolates of L. innocua and the remnants of the cluster in several others. Significantly, we can reveal that placing the L. innocua lls genes under the control of a constitutive promoter results in a haemolytic phenotype, confirming that the cluster is capable of encoding a functional haemolysin. Conclusions: Although the presence of the LIPI-3 gene cluster is confined to lineage I isolates of L. monocytogenes, a corresponding gene cluster or its remnants have been identified in many L. innocua strains.en
dc.description.sponsorshipEnterprise Ireland (Commercialisation Fund); European Commission (European Regional Development Fund); Science Foundation Ireland (Investigator awards (06/IN.1/B98) and (10/IN.1/B3027)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid58
dc.identifier.citationCLAYTON, E. M., DALY, K. M., GUINANE, C. M., HILL, C., COTTER, P. D. & ROSS, P. R. 2014. Atypical Listeria innocua strains possess an intact LIPI-3. BMC Microbiology, 14:58, 1-9. http://dx.doi.org/10.1186/1471-2180-14-58en
dc.identifier.doi10.1186/1471-2180-14-58
dc.identifier.endpage9en
dc.identifier.issn1471-2180
dc.identifier.journaltitleBMC Microbiologyen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/2233
dc.identifier.volume14en
dc.language.isoenen
dc.publisherBioMed Central Ltd.en
dc.rights© Clayton et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://​creativecommons.​org/​licenses/​by/​2.​0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.en
dc.rights.urihttp://​creativecommons.​org/​licenses/​by/​2.​0en
dc.subjectGenus Listeriaen
dc.subjectMonocytogenesen
dc.subjectVirulenceen
dc.subjectEvolutionen
dc.subjectPathogenesisen
dc.subjectGenomicsen
dc.subjectGenesen
dc.titleAtypical Listeria innocua strains possess an intact LIPI-3en
dc.typeArticle (peer-reviewed)en
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