Modification of Escherichia coli-bacteriophage interactions by surfactants and antibiotics in vitro

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dc.contributor.authorScanlan, Pauline D.
dc.contributor.authorBischofberger, Anna M.
dc.contributor.authorHall, Alex R.
dc.contributor.funderSchweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschungen
dc.contributor.funderFP7 People: Marie-Curie Actionsen
dc.contributor.funderSeventh Framework Programmeen
dc.date.accessioned2018-07-02T15:24:10Z
dc.date.available2018-07-02T15:24:10Z
dc.date.issued2016-12-10
dc.date.updated2018-07-01T21:18:30Z
dc.description.abstractAlthough experiments indicate that the abiotic environment plays an important role in bacterial interactions with their parasitic viruses (bacteriophages or phages), it is not yet clear how exposure to compounds present in nature alters the impact of phages on bacterial growth and evolution. To address this question, we exposed Escherichia coli K12 MG1655, in combination with three lytic phages, to various substances that natural and clinical microbial populations are likely to encounter: bile salts (present in mammalian gastrointestinal tracts), sodium dodecyl sulfate (SDS, a common surfactant in cleaning and hygiene products) and four antibiotics (present at variable concentrations in natural and clinical environments). Our results show that bile salts and SDS can reduce the detrimental effect of phages on bacterial growth. In some cases these compounds completely mitigated any negative effects of phages on bacterial growth and consequently bacteria did not evolve resistance to phages in these conditions. The proportional effects of phages were unaffected by antibiotics in most combinations, excepting three cases of phage-drug synergy. These results suggest that accounting for interactions between phages and environmental factors such as surfactants and antibiotics will improve understanding of both bacterial growth and resistance evolution to phages in vivo and in nature.en
dc.description.sponsorshipEuropean Commission (Marie Curie Intra-European Fellowships for Career Development (IEF))en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationScanlan, P. D., Bischofberger, A. M. and Hall, A. R. (2017) 'Modification of Escherichia coli–bacteriophage interactions by surfactants and antibiotics in vitro', FEMS Microbiology Ecology, 93(1), fiw211 (9 pp). doi: 10.1093/femsec/fiw211en
dc.identifier.doi10.1093/femsec/fiw211
dc.identifier.endpagefiw211-9en
dc.identifier.issn0168-6496
dc.identifier.issued1en
dc.identifier.journaltitleFems Microbiology Ecologyen
dc.identifier.startpagefiw211-1en
dc.identifier.urihttps://hdl.handle.net/10468/6403
dc.identifier.volume93en
dc.language.isoenen
dc.publisherOxford University Pressen
dc.relation.projectinfo:eu-repo/grantAgreement/SNSF/Careers::Ambizione/PZ00P3_148255/CH/Ecology of Antibiotic Resistance: Effect of Environment on Bacterial Adaptation to Antibiotics/en
dc.rights© FEMS 2016. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.en
dc.subjectBacteriophageen
dc.subjectAntibioticsen
dc.subjectBile saltsen
dc.subjectEscherichia colien
dc.subjectPhage therapyen
dc.subjectGenotype-by-environment interactionen
dc.subjectBacteriophagesen
dc.subjectPulmonary surfactantsen
dc.subjectBacteriaen
dc.subjectEscherichia colien
dc.subjectChemical surfactantsen
dc.subjectPhage therapyen
dc.subjectPseudomonas-aeruginosaen
dc.subjectEvolutionary dynamicsen
dc.subjectResistanceen
dc.subjectCoevolutionen
dc.subjectVirusesen
dc.subjectBileen
dc.subjectFluorescensen
dc.subjectPopulationen
dc.subjectMechanismsen
dc.titleModification of Escherichia coli-bacteriophage interactions by surfactants and antibiotics in vitroen
dc.typeArticle (peer-reviewed)en
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