Analytical method validation for assay determination of cannabidiol and tetrahydrocannabinol in hemp oil infused products by RP-HPLC

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dc.contributor.authorAnalakkattillam, Sandhyarani
dc.contributor.authorLangsi, Victor K.
dc.contributor.authorHanrahan, John P.
dc.contributor.authorMoore, Eric
dc.contributor.funderIrish Research Councilen
dc.date.accessioned2023-02-23T16:08:50Z
dc.date.available2023-02-23T16:08:50Z
dc.date.issued2022-07
dc.date.updated2023-02-23T15:53:33Z
dc.description.abstractA simple quantitative reverse phase high performance liquid chromatographic (RP-HPLC) method has been developed and validated for assay determination of cannabidiol and tetrahydrocannabinol in hemp oil infused products. The RP-HPLC method was developed and optimized for the mobile phase composition, flow rate, column selection and detector wavelength. An isocratic elution of samples were performed on SOLAS 100 angstrom C18 150 mm x 4.6 mm, 5 mu m column with a mobile phase containing 75/25 acetonitrile/water v/v, with a flow rate of 1.5 mL/min by using an ultraviolet-visible (UV/Vis) detector operating at 214 nm. The RP-HPLC method was validated to meet regulatory requirements which covers specificity, accuracy, range, linearity, precision, system suitability and robustness. The validated assay test method was applied successfully to quantify cannabidiol and tetrahydrocannabinol in commercial hemp oil infused products such as tablets, soft gel capsules, plant extract oils, oral drops, tincture, and beverage enhancers. All the test results were found acceptable as per ICH guidelines, and this confirmed the feasibility of this method for its intended use in regular quality control and assay of cannabidiol and tetrahydrocannabinol in hemp oil infused products.en
dc.description.sponsorshipIrish Research Council (Employment-Based postgraduate programme scholarship (Project ID: EBPPG/2019/38))en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid12453en
dc.identifier.citationAnalakkattillam, S., Langsi, V.K., Hanrahan, J.P. and Moore, E. (2022) ‘Analytical method validation for assay determination of cannabidiol and tetrahydrocannabinol in hemp oil infused products by RP-HPLC’, Scientific Reports, 12(1), 12453 (11pp). doi: 10.1038/s41598-022-13737-6en
dc.identifier.doi10.1038/s41598-022-13737-6en
dc.identifier.endpage11en
dc.identifier.issn2045-2322
dc.identifier.journaltitleScientific Reportsen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/14240
dc.identifier.volume12en
dc.language.isoenen
dc.publisherNature Researchen
dc.rights© The Author(s) 2022. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. Te images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectGas-chromatographyen
dc.subjectSimultaneous quantificationen
dc.subjectSpectrometry methoden
dc.subjectCannabinoidsen
dc.subjectDelta(9)-tetrahydrocannabinolen
dc.subjectHairen
dc.subjectAciden
dc.subjectTHCen
dc.subjectCBDen
dc.titleAnalytical method validation for assay determination of cannabidiol and tetrahydrocannabinol in hemp oil infused products by RP-HPLCen
dc.typeArticle (peer-reviewed)en
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