Towards an in-situ non-lethal rapid test to accurately detect the presence of the nematode parasite, Anguillicoloides crassus, in European eel, Anguilla anguilla

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dc.contributor.authorDe Noia, M.
dc.contributor.authorPoole, R.
dc.contributor.authorKaufmann, Joshka
dc.contributor.authorWaters, C.
dc.contributor.authorAdams, C.
dc.contributor.authorMcGinnity, Philip
dc.contributor.authorLlewellyn, M.
dc.contributor.funderBiotechnology and Biological Sciences Research Councilen
dc.contributor.funderScience Foundation Irelanden
dc.contributor.funderMarine Instituteen
dc.contributor.funderDepartment for the Economyen
dc.date.accessioned2022-01-25T14:11:41Z
dc.date.available2022-01-25T14:11:41Z
dc.date.issued2022-01-19
dc.date.updated2022-01-25T13:37:58Z
dc.description.abstractAnguillicoloides crassus is an invasive nematode parasite of the critically endangered European eel, Anguilla anguilla, and possibly one of the primary drivers of eel population collapse. The presence of the parasite has been shown to impact many features of eel physiology and life history. Early detection of the parasite is vital to limit the spread of A. crassus, and to assess its potential impact on spawning biomass. However, until recently, accurate diagnosis of infection could only be achieved via necropsy. To support A. anguilla fisheries management in the context of A. crassus we developed a rapid, non-lethal, minimally invasive and in-situ DNA-based method to infer the presence of the parasite in the swim bladder. Screening of 131 wild eels was undertaken between 2017 and 2019 in Ireland and UK to validate the procedure. DNA extractions and PCR were conducted using both a Qiagen Stool kit at Glasgow University and in situ using Whatman qualitative filter paper No. 1 and a miniPCR DNA Discovery Systemâ„¢. Primers were specifically designed to target the cytochrome oxidase mtDNA gene region and in situ extraction and amplification takes approximately 3h for up to 16 individuals. Our in situ diagnostic procedure demonstrated Positive Predictive Values at 96% and Negative Predictive Values at 87% by comparison to necropsy data. Our method could be a valuable tool in the hands of fisheries managers to enable infection control and help protect this iconic but critically endangered species.en
dc.description.sponsorshipBiotechnology and Biological Sciences Research Council (grant number BB/P001203/1); Science Foundation Ireland (FI/15/IA/3028); Marine Institute, Ireland (Beyond 2020 program)en
dc.description.statusPeer revieweden
dc.description.versionAccepted Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationDe Noia, M., Poole, R., Kaufmann, J., Waters, C., Adams, C., McGinnity, P. and Llewellyn, M. (2022) 'Towards an in-situ non-lethal rapid test to accurately detect the presence of the nematode parasite, Anguillicoloides crassus, in European eel, Anguilla anguilla', Parasitology, 149(5), pp. 605-611. doi: 10.1017/S0031182021002146en
dc.identifier.doi10.1017/S0031182021002146en
dc.identifier.endpage611
dc.identifier.issn1469-8161
dc.identifier.issued5
dc.identifier.journaltitleParasitologyen
dc.identifier.startpage605
dc.identifier.urihttps://hdl.handle.net/10468/12468
dc.identifier.volume149
dc.language.isoenen
dc.publisherCambridge University Pressen
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Investigator Programme/15/IA/3028/IE/Wild farmed interactions in a changing world: formulation of a predictive methodology to inform environmental best practice to secure long-term sustainability of global wild and farm fish populations/en
dc.rights© 2022, the Authors. Published by Cambridge University Press. This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted re-use, distribution and reproduction, provided the original article is properly cited.en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectNon-lethal testen
dc.subjectFish parasiteen
dc.subjectGeneticen
dc.subjecteDNAen
dc.titleTowards an in-situ non-lethal rapid test to accurately detect the presence of the nematode parasite, Anguillicoloides crassus, in European eel, Anguilla anguillaen
dc.typeArticle (peer-reviewed)en
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