Vancomycin and nisin A are effective against biofilms of multi-drug resistant Staphylococcus aureus isolates from human milk

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dc.contributor.authorAngelopoulou, Angeliki
dc.contributor.authorField, Des
dc.contributor.authorPérez-Ibarreche, Mariana
dc.contributor.authorWarda, Alicja K.
dc.contributor.authorHill, Colin
dc.contributor.authorRoss, R. Paul
dc.contributor.funderScience Foundation Irelanden
dc.contributor.funderAPC Microbiome Instituteen
dc.date.accessioned2023-02-16T12:51:35Z
dc.date.available2023-02-16T12:51:35Z
dc.date.issued2020-01
dc.date.updated2023-02-16T12:30:15Z
dc.description.abstractHuman milk provides complete nutrition for infants and at the same time promotes the growth of specific bacteria in the infant gastrointestinal tract. Breastfeeding can often be discontinued due to mastitis which is an inflammation of the breast tissue. We isolated 18 Staphylococcus aureus strains from milk donated by healthy (n = 6), subclinical (n = 6), and mastitic (n = 6) mothers, two strains of which were VISA (Vancomycin Intermediate S. aureus). All tested strains (n = 12) were able to form biofilms. We then examined the impact of nisin A and vancomycin alone and in combination on biofilm formation and eradication of selected strains (n = 8). We observed strain-specific responses, with the combinatorial treatment at 1/4X MIC (for both singularly) significantly inhibiting biofilm formation for seven out of eight strains when compared with nisin A or vancomycin alone. None of the selected treatments were able to eradicate pre-formed biofilms. Finally, we selected two strains, namely a VISA (APC3814H) and a strong biofilm former (APC3912CM) and used confocal microscopy to evaluate the effects of the antimicrobial agents at 1X MIC on biofilm inhibition and eradication. All treatments inhibited biofilm formation of APC3814H but were ineffective in eradicating a pre-formed biofilm. Single treatments at 1X MIC against APC3912CM cells did not prevent biofilm formation whereas combination treatment caused increased death of APC3912CM cells. Finally, the combination treatment reduced the thickness of the pre-formed APC3912CM biofilm as compared with the single treatments.en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleide0233284en
dc.identifier.citationAngelopoulou, A., Field, D., Pérez-Ibarreche, M., Warda, A.K., Hill, C. and Ross, R.P. (2020) ‘Vancomycin and nisin A are effective against biofilms of multi-drug resistant Staphylococcus aureus isolates from human milk’, PLOS ONE,15(5), e0233284 (19pp). doi: 10.1371/journal.pone.0233284en
dc.identifier.doi10.1371/journal.pone.0233284en
dc.identifier.endpage19en
dc.identifier.issn1932-6203
dc.identifier.issued5en
dc.identifier.journaltitlePlos Oneen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/14224
dc.identifier.volume15en
dc.language.isoenen
dc.publisherPLOSen
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2273/IE/Alimentary Pharmabiotic Centre (APC) - Interfacing Food & Medicine/en
dc.rights© 2020 Angelopoulou et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectBacterial biofilmsen
dc.subjectVancomycinen
dc.subjectStaphylococcus aureusen
dc.subjectMastitisen
dc.subjectAntibiotic resistanceen
dc.subjectAntimicrobialsen
dc.subjectAntibioticsen
dc.subjectMilken
dc.titleVancomycin and nisin A are effective against biofilms of multi-drug resistant Staphylococcus aureus isolates from human milken
dc.typeArticle (peer-reviewed)en
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