Serendipitous identification of natural intergenotypic recombinants of hepatitis C in Ireland

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dc.contributor.authorMoreau, Isabelle
dc.contributor.authorHegarty, Susan
dc.contributor.authorLevis, John
dc.contributor.authorSheehy, Patrick
dc.contributor.authorCrosbie, Orla
dc.contributor.authorKenny-Walsh, Elizabeth
dc.contributor.authorFanning, Liam J.
dc.date.accessioned2013-01-22T13:37:07Z
dc.date.available2013-01-22T13:37:07Z
dc.date.copyright2006
dc.date.issued2006-06
dc.date.updated2013-01-18T15:09:22Z
dc.description.abstractBackground: Recombination between hepatitis C single stranded RNA viruses is a rare event. Natural viable intragenotypic and intergenotypic recombinants between 1b-1a, 1a-1c and 2k-1b, 2i-6p, respectively, have been reported. Diagnostically recombinants represent an intriguing challenge. Hepatitis C genotype is defined by interrogation of the sequence composition of the 5' untranslated region [5' UTR]. Occasionally, ambiguous specimens require further investigation of the genome, usually by interrogation of the NS5B region. The original purpose of this study was to confirm the existence of a suspected mixed genotype infection of genotypes 2 and 4 by clonal analysis at the NS5B region of the genome in two specimens from two separate individuals. This initial identification of genotype was based on analysis of the 5' UTR of the genome by reverse line probe hybridisation [RLPH].Results: The original diagnosis of a mixed genotype infection was not confirmed by clonal analysis of the NS5B region of the genome. The phylogenetic analysis indicated that both specimens were natural intergenotypic recombinant forms of HCV. The recombination was between genotypes 2k and 1b for both specimens. The recombination break point was identified as occurring within the NS2 region of the genome. Conclusion: The viral recombinants identified here resemble the recombinant form originally identified in Russia. The RLPH pattern observed in this study may be a signature indicative of this particular type of intergenotype recombinant of hepatitis C meriting clonal analysis of NS2.en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationMoreau, I., Hegarty, S., Levis, J., Sheehy, P., Crosbie, O., Kenny-Walsh, E.,Fanning, L.J. (2006) 'Serendipitous identification of natural Intergenotypic recombinants of hepatitis C in Ireland'. Virology Journal, 3: 95. http://dx.doi.org/10.1186/1743-422X-3-95en
dc.identifier.doi10.1186/1743-422X-3-95
dc.identifier.issn1743-422X
dc.identifier.issued95en
dc.identifier.journaltitleVirology Journalen
dc.identifier.urihttps://hdl.handle.net/10468/909
dc.identifier.volume3en
dc.language.isoenen
dc.publisherBioMed Centralen
dc.relation.urihttp://www.virologyj.com/content/3/1/95
dc.rights© 2006 Moreau et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.en
dc.rights.urihttp://creativecommons.org/licenses/by/2.0en
dc.subjectSt Petersburgen
dc.subjectVirusen
dc.subjectSequenceen
dc.subjectInfectionen
dc.subjectGenotypeen
dc.subjectPatternsen
dc.subjectSubtypesen
dc.subjectRegionen
dc.subjectFranceen
dc.subjectIrelanden
dc.subject.lcshHepatitis C--Irelanden
dc.titleSerendipitous identification of natural intergenotypic recombinants of hepatitis C in Irelanden
dc.typeArticle (peer-reviewed)en
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