Bifidobacterium transformation

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dc.contributor.authorHoedt, Emily C.
dc.contributor.authorBongers, Roger S.
dc.contributor.authorBottacini, Francesca
dc.contributor.authorKnol, Jan
dc.contributor.authorMacSharry, John
dc.contributor.authorvan Sinderen, Douwe
dc.contributor.editorvan Sinderen, Douwe
dc.contributor.editorVentura, Marco
dc.contributor.funderNutricia Research Foundationen
dc.contributor.funderScience Foundation Irelanden
dc.date.accessioned2021-03-19T12:27:39Z
dc.date.available2021-03-19T12:27:39Z
dc.date.issued03/03/2021
dc.date.updated2021-03-19T12:11:37Z
dc.description.abstractThe protocol presented in this chapter describes a generic method for electrotransformation of Bifidobacterium spp., outlining a technique that is ideal for conferring selective properties onto strains as well as allowing the user to introduce or knock out/in selected genes for phenotypic characterization purposes. We have generalized on the plasmid chosen for transformation and antibiotic selection marker, but the protocol is versatile in this respect and we are able to achieve transformation efficiencies up to 107 transformants/μg of DNA.en
dc.description.sponsorshipScience Foundation Ireland (Grant Numbers SFI/12/RC/2273-P1; SFI/12/RC/2273-P2)en
dc.description.statusPeer revieweden
dc.description.versionAccepted Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationHoedt, Emily C.; Bongers, Roger S.; Bottacini, Francesca; Knol, Jan; MacSharry, John; van Sinderen, Douwe (2021) 'Bifidobacterium transformation', in van Sinderen, D. and Ventura, M. (eds) Bifidobacteria - Methods and Protocols. Methods in Molecular Biology, vol 2278, pp. 13-19. New York, NY: Humana Press. doi: 10.1007/978-1-0716-1274-3_2en
dc.identifier.doi10.1007/978-1-0716-1274-3_2en
dc.identifier.endpage19en
dc.identifier.isbn978-1-0716-1273-6
dc.identifier.isbn978-1-0716-1274-3
dc.identifier.issn1064-3745
dc.identifier.journaltitleMethods in Molecular Biologyen
dc.identifier.startpage13en
dc.identifier.urihttps://hdl.handle.net/10468/11151
dc.identifier.volume2278en
dc.language.isoenen
dc.publisherSpringer Science+Business Media, LLCen
dc.relation.ispartofBifidobacteria - Methods and Protocols
dc.relation.ispartofMethods in Molecular Biology, vol 2278
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2273/IE/Alimentary Pharmabiotic Centre (APC) - Interfacing Food & Medicine/en
dc.rights© 2021, Springer Science+Business Media, LLC, part of Springer Nature. This is a post-peer-review, pre-copyedit version of a chapter published in van Sinderen, D. and Ventura, M. (eds) Bifidobacteria - Methods and Protocols. Methods in Molecular Biology, vol 2278, pp. 13-19. New York, NY: Humana Press. The final authenticated version is available online at: https://doi.org/10.1007/978-1-0716-1274-3_2en
dc.subjectBifidobacterialen
dc.subjectElectroporationen
dc.subjectGenetic accessibilityen
dc.subjectProbioticen
dc.titleBifidobacterium transformationen
dc.typeBook chapteren
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