Integrated phenotypic-genotypic approach to understand the influence of ultrasound on metabolic response of Lactobacillus sakei

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dc.contributor.authorOjha, K. Shikha
dc.contributor.authorBurgess, Catherine M.
dc.contributor.authorDuffy, Geraldine
dc.contributor.authorKerry, Joseph P.
dc.contributor.authorTiwari, Brijesh K.
dc.contributor.funderTeagasc
dc.date.accessioned2018-02-20T13:24:14Z
dc.date.available2018-02-20T13:24:14Z
dc.date.issued2018
dc.description.abstractThe lethal effects of soundwaves on a range of microorganisms have been known for almost a century whereas, the use of ultrasound to promote or control their activity is much more recent. Moreover, the fundamental molecular mechanism influencing the behaviour of microorganisms subjected to ultrasonic waves is not well established. In this study, we investigated the influence of ultrasonic frequencies of 20, 45, 130 and 950 kHz on growth kinetics of Lactobacillus sakei. A significant increase in the growth rate of L. sakei was observed following ultrasound treatment at 20 kHz despite the treatment yielding a significant reduction of ca. 3 log cfu/mL in cells count. Scanning electron microscopy showed that ultrasound caused significant changes on the cell surface of L. sakei culture with the formation of pores “sonoporation”. Phenotypic microarrays showed that all ultrasound treated L. sakei after exposure to various carbon, nitrogen, phosphorus and sulphur sources had significant variations in nutrient utilisation. Integration of this phenotypic data with the genome of L. sakei revealed that various metabolic pathways were being influenced by the ultrasound treatments. Results presented in this study showed that the physiological response of L. sakei in response to US is frequency dependent and that it can influence metabolic pathways. Hence, ultrasound treatments can be employed to modulate microbial activity for specialised applications.en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleide0191053
dc.identifier.citationOjha, K. S., Burgess, C. M., Duffy, G., Kerry, J. P. and Tiwari, B. K. (2018) 'Integrated phenotypic-genotypic approach to understand the influence of ultrasound on metabolic response of Lactobacillus sakei', PLOS ONE, 13(1), e0191053 (20pp). doi: 10.1371/journal.pone.0191053en
dc.identifier.doi10.1371/journal.pone.0191053
dc.identifier.endpage20
dc.identifier.issn1932-6203
dc.identifier.issued1
dc.identifier.journaltitlePLOS ONEen
dc.identifier.startpage1
dc.identifier.urihttps://hdl.handle.net/10468/5516
dc.identifier.volume13
dc.language.isoenen
dc.publisherPublic Library of Scienceen
dc.relation.urihttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0191053
dc.rights© 2018, Ojha et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectRiboseen
dc.subjectMetabolic pathwaysen
dc.subjectGenomicsen
dc.subjectNitrogen metabolismen
dc.subjectSulfuren
dc.subjectCell membranesen
dc.subjectSound pressureen
dc.subjectPentosesen
dc.titleIntegrated phenotypic-genotypic approach to understand the influence of ultrasound on metabolic response of Lactobacillus sakeien
dc.typeArticle (peer-reviewed)en
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