Electrochemical detection of Pseudomonas aeruginosa quorum sensing molecules at a liquid|liquid interface

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dc.contributor.authorBurgoyne, Edward D.
dc.contributor.authorStockmann, Talia Jane
dc.contributor.authorMolina-Osorio, Andrés F.
dc.contributor.authorShanahan, Rachel
dc.contributor.authorMcGlacken, Gerard P.
dc.contributor.authorScanlon, Micheál D.
dc.contributor.funderEuropean Research Councilen
dc.contributor.funderScience Foundation Irelanden
dc.contributor.funderIrish Research Councilen
dc.contributor.funderNatural Sciences and Engineering Research Council of Canadaen
dc.date.accessioned2019-09-18T09:12:18Z
dc.date.available2019-09-18T09:12:18Z
dc.date.issued2019-09-12
dc.date.updated2019-09-18T08:55:20Z
dc.description.abstractOpportunistic pathogenic bacteria, such as Pseudomonas aeruginosa, pose a serious risk to patients suffering from a compromised immune system and those patients with cystic fibrosis. Confirming their presence often requires culturing the bacteria which can take days. Herein is proposed a rapid electrochemical detection method based on P. aeruginosa generated small molecules employed for chemical communication – referred to as quorum sensing (QS) molecules – within the biofilm formed by the bacteria; specifically, 4‐hydroxy‐2‐heptylquinoline (HHQ) and 2‐heptyl‐3,4‐dihydroxyquinoline (PQS, Pseudomonas quinolone signal). This method does not depend on the redox activity of the QS molecules. Instead, as a proof-of-concept, electrochemical monitoring was achieved through aqueous alkali metal ion and proton interfacial complexation with organic solubilized HHQ and PQS at an interface between two immiscible electrolyt-ic solutions (ITIES), specifically, between water and 1,2-dichloroethane. The proton:HHQ and proton:PQS binding stoi-chiometry’s were discovered to be 1:3 and 1:2, respectively, which is likely due to the relatively high concentrations of QS molecules employed. Owing to the biphasic nature of the methodology, experimental complications due to the poor solubility of the hydrophobic QS molecules in aqueous media were avoided.en
dc.description.sponsorshipEuropean Research Council (Grant Agreement no. 716792); Irish Research Council (Government of Ireland Postgraduate Scholarship Award - Grant Number GOIPG/2016/1217); Natural Sciences and Engineering Research Council of Canada (Discovery Grant #2019-06074 and Discovery Launch Supplement)en
dc.description.statusPeer revieweden
dc.description.versionAccepted Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationBurgoyne, E. D., Stockmann, T. J., Molina-Osorio, A. F., Shanahan, R., McGlacken, G. P. and Scanlon, M. D. (2019) 'Electrochemical detection of Pseudomonas aeruginosa quorum sensing molecules at a liquid|liquid interface', Journal of Physical Chemistry C. doi: 10.1021/acs.jpcc.9b08350en
dc.identifier.doi10.1021/acs.jpcc.9b08350en
dc.identifier.eissn1932-7455
dc.identifier.endpage15en
dc.identifier.issn1932-7447
dc.identifier.journaltitleJournal of Physical Chemistry Cen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/8559
dc.language.isoenen
dc.publisherAmerican Chemical Societyen
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Starting Investigator Research Grant (SIRG)/13/SIRG/2137/IE/Designing Reactive Functionalised Soft Interfaces _ Self-healing soft materials for solar energy conversion, energy storage, and sustainable low cost hydrogen production/en
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Investigator Programme/12/IP/1315/IE/The Direct Arylation of Pyrones, Coumarins, Pyridones and Quinolones/en
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Technology and Innovation Development Award (TIDA)/12/TIDA/B2405/IE/Optimised detection of key biomarkers of Pseudomonas aeruginosa towards a clinical application/en
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2275/IE/Synthesis and Solid State Pharmaceutical Centre (SSPC)/en
dc.rights© 2019, American Chemical Society. This document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of Physical Chemistry C, © American Chemical Society, after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acs.jpcc.9b08350en
dc.subjectImmune systemen
dc.subjectCystic fibrosisen
dc.subjectRapid electrochemical detectionen
dc.subjectChemical communicationen
dc.subjectQuorum sensing moleculesen
dc.subjectBiofilmen
dc.titleElectrochemical detection of Pseudomonas aeruginosa quorum sensing molecules at a liquid|liquid interfaceen
dc.typeArticle (peer-reviewed)en
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