Pectobacterium atrosepticum phage vB_PatP_CB5: A member of the proposed genus ‘Phimunavirus’
| dc.contributor.author | Buttimer, Colin | |
| dc.contributor.author | Lucid, Alan | |
| dc.contributor.author | Neve, Horst | |
| dc.contributor.author | Franz, Charles M. A. P. | |
| dc.contributor.author | O'Mahony, Jim | |
| dc.contributor.author | Turner, Dann | |
| dc.contributor.author | Lavigne, Rob | |
| dc.contributor.author | Coffey, Aidan | |
| dc.contributor.funder | Science Foundation Ireland | en |
| dc.contributor.funder | KU Leuven | en |
| dc.date.accessioned | 2018-09-27T12:08:27Z | |
| dc.date.available | 2018-09-27T12:08:27Z | |
| dc.date.issued | 2018 | |
| dc.description.abstract | Pectobacterium atrosepticum is a phytopathogen of economic importance as it is the causative agent of potato blackleg and soft rot. Here we describe the Pectobacterium phage vB_PatP_CB5 (abbreviated as CB5), which specifically infects the bacterium. The bacteriophage is characterized in detail and TEM micrographs indicate that it belongs to the Podoviridae family. CB5 shares significant pairwise nucleotide identity (≥80%) with P. atrosepticum phages φM1, Peat1, and PP90 and also shares common genome organization. Phylograms constructed using conserved proteins and whole-genome comparison-based amino acid sequences show that these phages form a distinct clade within the Autographivirinae. They also possess conserved RNA polymerase recognition and specificity loop sequences. Their lysis cassette resembles that of KP34virus, containing in sequential order a U-spanin, a holin, and a signal–arrest–release (SAR) endolysin. However, they share low pairwise nucleotide identity with the type phage of the KP34virus genus, Klebsiella phage KP34. In addition, phage KP34 does not possess several conserved proteins associated with these P. atrosepticum phages. As such, we propose the allocation of phages CB5, Peat1, φM1, and PP90 to a separate new genus designated Phimunavirus. | en |
| dc.description.sponsorship | KU Leuven(GOA grant “Phage Biosystems”). | en |
| dc.description.status | Peer reviewed | en |
| dc.description.version | Published Version | en |
| dc.format.mimetype | application/pdf | en |
| dc.identifier.articleid | 19 | |
| dc.identifier.citation | Buttimer, C., Lucid, A., Neve, H., Franz, C., O’Mahony, J., Turner, D., Lavigne, R. and Coffey, A. (2018) 'Pectobacterium atrosepticum phage vB_PatP_CB5: A member of the proposed genus ‘Phimunavirus’', Viruses, 10(8), 394 (19pp). doi: 10.3390/v10080394 | en |
| dc.identifier.doi | 10.3390/v10080394 | |
| dc.identifier.endpage | 19 | |
| dc.identifier.issn | 1999-4915 | |
| dc.identifier.issued | 8 | |
| dc.identifier.journaltitle | Viruses | en |
| dc.identifier.startpage | 1 | |
| dc.identifier.uri | https://hdl.handle.net/10468/6951 | |
| dc.identifier.volume | 10 | |
| dc.language.iso | en | en |
| dc.publisher | MDPI AG | en |
| dc.relation.project | info:eu-repo/grantAgreement/SFI/SFI Research Infrastructure Programme/12/RI/2335 (3)/IE/MALDI-TOF mass spectrometer/ | |
| dc.relation.uri | http://www.mdpi.com/1999-4915/10/8/394 | |
| dc.rights | © 2018, by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). | en |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
| dc.subject | Pectobacterium atrosepticum | en |
| dc.subject | Autographivirinae | en |
| dc.subject | ‘Phimunavirus’ | en |
| dc.subject | Bacteriophage | en |
| dc.subject | Phage | en |
| dc.subject | Podoviridae | en |
| dc.title | Pectobacterium atrosepticum phage vB_PatP_CB5: A member of the proposed genus ‘Phimunavirus’ | en |
| dc.type | Article (peer-reviewed) | en |
