Allelic variation of bile salt hydrolase genes in Lactobacillus salivarius does not determine bile resistance levels

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dc.contributor.author Fang, Fang
dc.contributor.author Li, Yin
dc.contributor.author Bumann, Mario
dc.contributor.author Raftis, Emma J.
dc.contributor.author Casey, Pat G.
dc.contributor.author Cooney, Jakki C.
dc.contributor.author Walsh, Martin A.
dc.contributor.author O'Toole, Paul W.
dc.date.accessioned 2010-03-15T11:50:01Z
dc.date.available 2010-03-15T11:50:01Z
dc.date.issued 2009-09
dc.identifier.citation Fang F., Li Y., Bumann M., Raftis E.J., Casey P.G., Cooney J.C., Walsh M.A., O'Toole P.W. 2009. Allelic variation of bile salt hydrolase genes in Lactobacillus salivarius does not determine bile resistance levels. Journal of Bacteriology 191(18):5743-57. en
dc.identifier.volume 191 en
dc.identifier.issued 18 en
dc.identifier.startpage 5743 en
dc.identifier.endpage 5757 en
dc.identifier.issn 0021-9193
dc.identifier.issn 1098-5530
dc.identifier.uri http://hdl.handle.net/10468/120
dc.identifier.doi 10.1128/JB.00506-09
dc.description.abstract Commensal lactobacilli frequently produce bile salt hydrolase (Bsh) enzymes whose roles in intestinal survival are unclear. Twenty-six Lactobacillus salivarius strains from different sources all harbored a bsh1 allele on their respective megaplasmids. This allele was related to the plasmid-borne bsh1 gene of the probiotic strain UCC118. A second locus (bsh2) was found in the chromosomes of two strains that had higher bile resistance levels. Four Bsh1-encoding allele groups were identified, defined by truncations or deletions involving a conserved residue. In vitro analyses showed that this allelic variation was correlated with widely varying bile deconjugation phenotypes. Despite very low activity of the UCC118 Bsh1 enzyme, a mutant lacking this protein had significantly lower bile resistance, both in vitro and during intestinal transit in mice. However, the overall bile resistance phenotype of this and other strains was independent of the bsh1 allele type. Analysis of the L. salivarius transcriptome upon exposure to bile and cholate identified a multiplicity of stress response proteins and putative efflux proteins that appear to broadly compensate for, or mask, the effects of allelic variation of bsh genes. Bsh enzymes with different bile-degrading kinetics, though apparently not the primary determinants of bile resistance in L. salivarius, may have additional biological importance because of varying effects upon bile as a signaling molecule in the host. en
dc.description.sponsorship Science Foundation Ireland (Centre for Science, Engineering and Technology). en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher American Society for Microbiology en
dc.relation.uri http://jb.asm.org/cgi/content/full/191/18/5743/DC1
dc.rights © American Society for Microbiology en
dc.subject Bile salt hydrolase enzyme en
dc.subject.lcsh Lactobacillus -- Genetics en
dc.subject.lcsh Bile en
dc.title Allelic variation of bile salt hydrolase genes in Lactobacillus salivarius does not determine bile resistance levels en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Pat G. Casey, Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland. E-mail: P.Casey@ucc.ie en
dc.internal.authorcontactother Paul W. O'Toole, Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland. E-mail: pwotoole@ucc.ie en
dc.internal.availability Full text available en
dc.description.version Accepted Version en
dc.contributor.funder Science Foundation Ireland en
dc.description.status Peer reviewed en
dc.identifier.journaltitle Journal of Bacteriology en
dc.internal.IRISemailaddress p.casey@ucc.ie en


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