Atypical Listeria innocua strains possess an intact LIPI-3

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dc.contributor.author Clayton, Evelyn M.
dc.contributor.author Daly, Karen M.
dc.contributor.author Guinane, Caitriona M.
dc.contributor.author Hill, Colin
dc.contributor.author Cotter, Paul D.
dc.contributor.author Ross, R. Paul
dc.date.accessioned 2016-01-29T13:24:03Z
dc.date.available 2016-01-29T13:24:03Z
dc.date.issued 2014-03-08
dc.identifier.citation CLAYTON, E. M., DALY, K. M., GUINANE, C. M., HILL, C., COTTER, P. D. & ROSS, P. R. 2014. Atypical Listeria innocua strains possess an intact LIPI-3. BMC Microbiology, 14:58, 1-9. http://dx.doi.org/10.1186/1471-2180-14-58 en
dc.identifier.volume 14 en
dc.identifier.startpage 1 en
dc.identifier.endpage 9 en
dc.identifier.issn 1471-2180
dc.identifier.uri http://hdl.handle.net/10468/2233
dc.identifier.doi 10.1186/1471-2180-14-58
dc.description.abstract Background: Listeria monocytogenes is a food-borne pathogen which is the causative agent of listeriosis and can be divided into three evolutionary lineages I, II and III. While all strains possess the well established virulence factors associated with the Listeria pathogenicity island I (LIPI-1), lineage I strains also possess an additional pathogenicity island designated LIPI-3 which encodes listeriolysin S (LLS), a post-translationally modified cytolytic peptide. Up until now, this pathogenicity island has been identified exclusively in a subset of lineage I isolates of the pathogen Listeria monocytogenes. Results: In total 64 L. innocua strains were screened for the presence of LIPI-3. Here we report the identification of an intact LIPI-3 in 11 isolates of L. innocua and the remnants of the cluster in several others. Significantly, we can reveal that placing the L. innocua lls genes under the control of a constitutive promoter results in a haemolytic phenotype, confirming that the cluster is capable of encoding a functional haemolysin. Conclusions: Although the presence of the LIPI-3 gene cluster is confined to lineage I isolates of L. monocytogenes, a corresponding gene cluster or its remnants have been identified in many L. innocua strains. en
dc.description.sponsorship Enterprise Ireland (Commercialisation Fund); European Commission (European Regional Development Fund); Science Foundation Ireland (Investigator awards (06/IN.1/B98) and (10/IN.1/B3027) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher BioMed Central Ltd. en
dc.rights © Clayton et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://​creativecommons.​org/​licenses/​by/​2.​0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. en
dc.rights.uri http://​creativecommons.​org/​licenses/​by/​2.​0 en
dc.subject Genus Listeria en
dc.subject Monocytogenes en
dc.subject Virulence en
dc.subject Evolution en
dc.subject Pathogenesis en
dc.subject Genomics en
dc.subject Genes en
dc.title Atypical Listeria innocua strains possess an intact LIPI-3 en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother R. Paul Ross, College of Science, Engineering and Food Science, University College Cork, Cork, Ireland. +353-21-490-3000 Email: p.ross@ucc.ie en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.contributor.funder Enterprise Ireland en
dc.contributor.funder Science Foundation Ireland en
dc.contributor.funder European Commission
dc.description.status Peer reviewed en
dc.identifier.journaltitle BMC Microbiology en
dc.internal.IRISemailaddress p.ross@ucc.ie en
dc.identifier.articleid 58


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© Clayton et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://​creativecommons.​org/​licenses/​by/​2.​0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. Except where otherwise noted, this item's license is described as © Clayton et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://​creativecommons.​org/​licenses/​by/​2.​0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.
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