Pro-inflammatory flagellin proteins of prevalent motile commensal bacteria are variably abundant in the intestinal microbiome of elderly humans

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dc.contributor.author Neville, B. Anne
dc.contributor.author Sheridan, Paul O.
dc.contributor.author Harris, Hugh Michael B.
dc.contributor.author Coughlan, Simone
dc.contributor.author Flint, Harry J.
dc.contributor.author Duncan, Sylvia H.
dc.contributor.author Jeffery, Ian B.
dc.contributor.author Claesson, Marcus J.
dc.contributor.author Ross, R. Paul
dc.contributor.author Scott, Karen P.
dc.contributor.author O'Toole, Paul W.
dc.date.accessioned 2016-02-17T11:45:33Z
dc.date.available 2016-02-17T11:45:33Z
dc.date.issued 2013
dc.identifier.citation Neville BA, Sheridan PO, Harris HMB, Coughlan S, Flint HJ, Duncan SH, et al. (2013) Pro-Inflammatory Flagellin Proteins of Prevalent Motile Commensal Bacteria Are Variably Abundant in the Intestinal Microbiome of Elderly Humans. PLoS ONE 8(7): e68919. doi:10.1371/journal.pone.0068919 en
dc.identifier.volume 8 en
dc.identifier.issued 7 en
dc.identifier.issn 1932-6203
dc.identifier.uri http://hdl.handle.net/10468/2370
dc.identifier.doi 10.1371/journal.pone.0068919
dc.description.abstract Some Eubacterium and Roseburia species are among the most prevalent motile bacteria present in the intestinal microbiota of healthy adults. These flagellate species contribute "cell motility" category genes to the intestinal microbiome and flagellin proteins to the intestinal proteome. We reviewed and revised the annotation of motility genes in the genomes of six Eubacterium and Roseburia species that occur in the human intestinal microbiota and examined their respective locus organization by comparative genomics. Motility gene order was generally conserved across these loci. Five of these species harbored multiple genes for predicted flagellins. Flagellin proteins were isolated from R. inulinivorans strain A2-194 and from E. rectale strains A1-86 and M104/1. The amino-termini sequences of the R. inulinivorans and E. rectale A1-86 proteins were almost identical. These protein preparations stimulated secretion of interleukin-8 (IL-8) from human intestinal epithelial cell lines, suggesting that these flagellins were pro-inflammatory. Flagellins from the other four species were predicted to be pro-inflammatory on the basis of alignment to the consensus sequence of pro-inflammatory flagellins from the beta- and gamma-proteobacteria. Many fliC genes were deduced to be under the control of sigma(28). The relative abundance of the target Eubacterium and Roseburia species varied across shotgun metagenomes from 27 elderly individuals. Genes involved in the flagellum biogenesis pathways of these species were variably abundant in these metagenomes, suggesting that the current depth of coverage used for metagenomic sequencing (3.13-4.79 Gb total sequence in our study) insufficiently captures the functional diversity of genomes present at low (<= 1%) relative abundance. E. rectale and R. inulinivorans thus appear to synthesize complex flagella composed of flagellin proteins that stimulate IL-8 production. A greater depth of sequencing, improved evenness of sequencing and improved metagenome assembly from short reads will be required to facilitate in silico analyses of complete complex biochemical pathways for low-abundance target species from shotgun metagenomes. en
dc.description.sponsorship Science Foundation Ireland (SFI Principal Investigator Award (07/IN.1/B1780), SFI Award to Alimentary Pharmabiotic Centre (APC)); Irish Research Council for Science Engineering and Technology (Embark studentship); Government of Ireland National Development Plan, Department of Agriculture Food and Marine, Health Research Board (FHRI award to the ELDERMET project); Scottish Government Rural and Environment Science and Analytical Service Division (RESAS), APC University College Cork (Studentship) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Public Library of Science en
dc.rights © 2013 Neville et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited en
dc.rights.uri http://creativecommons.org/licenses/by/4.0/ en
dc.subject Butyrate producing bacteria en
dc.subject Toll-like receptors en
dc.subject Human gut en
dc.subject Bacillus subtilis en
dc.subject Gene expression en
dc.subject Escherichia coli en
dc.subject Translation initiation en
dc.subject Human feces en
dc.subject Sequence en
dc.title Pro-inflammatory flagellin proteins of prevalent motile commensal bacteria are variably abundant in the intestinal microbiome of elderly humans en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Paul W. O' Toole, Microbiology, University College Cork, Cork, Ireland. +353-21-490-3000 Email: pwotoole@ucc.ie en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.internal.rssid 243941126
dc.internal.wokid WOS:000325211000062
dc.contributor.funder Science Foundation Ireland en
dc.contributor.funder Irish Research Council for Science Engineering and Technology en
dc.contributor.funder Health Research Board en
dc.contributor.funder Department of Agriculture, Food and the Marine, Ireland en
dc.contributor.funder University College Cork en
dc.description.status Peer reviewed en
dc.identifier.journaltitle PLOS ONE en
dc.internal.IRISemailaddress pwotoole@ucc.ie en
dc.identifier.articleid e68919


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© 2013 Neville et al.  This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Except where otherwise noted, this item's license is described as © 2013 Neville et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
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