Comparative genomic analyses of Mycoplasma hyopneumoniae pathogenic 168 strain and its high-passaged attenuated strain

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dc.contributor.author Liu, Wei
dc.contributor.author Xiao, Shaobo
dc.contributor.author Li, Mao
dc.contributor.author Guo, Shaohua
dc.contributor.author Li, Sha
dc.contributor.author Luo, Rui
dc.contributor.author Feng, Zhixin
dc.contributor.author Li, Bin
dc.contributor.author Zhou, Zhemin
dc.contributor.author Shao, Guoqing
dc.contributor.author Chen, Huanchun
dc.contributor.author Fang, Liurong
dc.date.accessioned 2016-02-25T17:16:20Z
dc.date.available 2016-02-25T17:16:20Z
dc.date.issued 2013-02-05
dc.identifier.citation LIU, W., XIAO, S., LI, M., GUO, S., LI, S., LUO, R., FENG, Z., LI, B., ZHOU, Z., SHAO, G., CHEN, H. & FANG, L. 2013. Comparative genomic analyses of Mycoplasma hyopneumoniae pathogenic 168 strain and its high-passaged attenuated strain. BMC Genomics, 14:80, 1-13. http://dx.doi.org/10.1186/1471-2164-14-80 en
dc.identifier.volume 14 en
dc.identifier.startpage 1 en
dc.identifier.endpage 13 en
dc.identifier.issn 1471-2164
dc.identifier.uri http://hdl.handle.net/10468/2402
dc.identifier.doi 10.1186/1471-2164-14-80
dc.description.abstract Background: Mycoplasma hyopneumoniae is the causative agent of porcine enzootic pneumonia (EP), a mild, chronic pneumonia of swine. Despite presenting with low direct mortality, EP is responsible for major economic losses in the pig industry. To identify the virulence-associated determinants of M. hyopneumoniae, we determined the whole genome sequence of M. hyopneumoniae strain 168 and its attenuated high-passage strain 168-L and carried out comparative genomic analyses. Results: We performed the first comprehensive analysis of M. hyopneumoniae strain 168 and its attenuated strain and made a preliminary survey of coding sequences (CDSs) that may be related to virulence. The 168-L genome has a highly similar gene content and order to that of 168, but is 4,483 bp smaller because there are 60 insertions and 43 deletions in 168-L. Besides these indels, 227 single nucleotide variations (SNVs) were identified. We further investigated the variants that affected CDSs, and compared them to reported virulence determinants. Notably, almost all of the reported virulence determinants are included in these variants affected CDSs. In addition to variations previously described in mycoplasma adhesins (P97, P102, P146, P159, P216, and LppT), cell envelope proteins (P95), cell surface antigens (P36), secreted proteins and chaperone protein (DnaK), mutations in genes related to metabolism and growth may also contribute to the attenuated virulence in 168-L. Furthermore, many mutations were located in the previously described repeat motif, which may be of primary importance for virulence. Conclusions: We studied the virulence attenuation mechanism of M. hyopneumoniae by comparative genomic analysis of virulent strain 168 and its attenuated high-passage strain 168-L. Our findings provide a preliminary survey of CDSs that may be related to virulence. While these include reported virulence-related genes, other novel virulence determinants were also detected. This new information will form the foundation of future investigations into the pathogenesis of M. hyopneumoniae and facilitate the design of new vaccines. en
dc.description.sponsorship National Natural Science Foundation of China (31170160, 31001080, and 31001066) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher BioMed Central Ltd. en
dc.rights © Liu et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://​creativecommons.​org/​licenses/​by/​2.​0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. en
dc.rights.uri http://​creativecommons.​org/​licenses/​by/​2.​0 en
dc.subject Mycoplasma hyopneumoniae en
dc.subject Genetic variation en
dc.subject Virulence attenuation en
dc.subject Sequence analysis en
dc.subject Repetitive sequences en
dc.subject Virulence factors en
dc.title Comparative genomic analyses of Mycoplasma hyopneumoniae pathogenic 168 strain and its high-passaged attenuated strain en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Zhemin Zhou, Environmental Research Institute, University College Cork, Cork, Ireland. +353-21-490-1931 Email: eri@ucc.ie en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.contributor.funder National Natural Science Foundation of China
dc.description.status Peer reviewed en
dc.identifier.journaltitle BMC Genomics en
dc.identifier.articleid 80


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© Liu et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://​creativecommons.​org/​licenses/​by/​2.​0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Except where otherwise noted, this item's license is described as © Liu et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://​creativecommons.​org/​licenses/​by/​2.​0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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