In vitro antioxidant and immunomodulatory activity of transglutaminase-treated sodium caseinate hydrolysates

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In vitro antioxidant and immunomodulatory activity of transglutaminase-treated sodium caseinate hydrolysates

Cermeño, Maria; FitzGerald, Richard J.; O'Brien, Nora M.
Date: 2016-08-31
Copyright: © 2016, Elsevier B.V. This manuscript version is made available under the CC-BY-NC-ND 4.0 license.
Copyright information: http://creativecommons.org/licenses/by-nc-nd/4.0/
Full text restriction information: Access to this article is restricted until 12 months after publication by request of the publisher.
Restriction lift date: 2017-08-31
Citation: Cermeño, M., FitzGerald, R.J. and O’Brien, N.M. (2016) 'In vitro antioxidant and immunomodulatory activity of transglutaminase-treated sodium caseinate hydrolysates', International Dairy Journal, 63, pp. 107-114. doi: 10.1016/j.idairyj.2016.08.007

Abstract:

Sodium caseinate (NaCN) was incubated prior to and after hydrolysis with a microbial transglutaminase (TGase) and hydrolysed with Prolyve 1000. The resultant hydrolysates were tested for their immunomodulatory and antioxidant activity. TGase-treated hydrolysates significantly reduced (p < 0.05) the production of IL-6 at 0.5 and 1 mg mL−1 and the non-TGase treated hydrolysate reduced the production of IL-6 at 1 mg mL−1 in concanavalin (ConA) stimulated Jurkat T cells. None of the samples had an effect on IL-2. The hydrolysates showed higher oxygen radical absorbance capacity assay and ferric reducing antioxidant power activity than unhydrolysed NaCN, but no significant (p > 0.05) differences were found between the TGase-treated and non-TGase-treated samples. In the presence of hydrogen peroxide, the non-TGase-treated sample exhibited the highest DNA protective effect in U937 cells. These findings suggest that NaCN derived hydrolysates with and without treatment with TGase may exert specific antioxidant, genoprotective and anti-inflammatory effects.

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© 2016, Elsevier B.V. This manuscript version is made available under the CC-BY-NC-ND 4.0 license. Except where otherwise noted, this item's license is described as © 2016, Elsevier B.V. This manuscript version is made available under the CC-BY-NC-ND 4.0 license.
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