Ruthenibacterium lactatiformans gen. nov., sp.nov., an anaerobic, lactate-producing member of the family Ruminococcaceae isolated from human faeces
Shkoporov, Andrei N.; Chaplin, Andrei V; Shcherbakova, Victoria A.; Suzina, Natalia E.; Kafarskaia, Lyudmila I.; Bozhenko, Vladimir K.; Efimov, Boris A.
Date:
2016-08-01
Copyright:
© 2016 IUMS. This is the Accepted Paper version of the following article: Shkoporov et al. Int J Syst Evol Microbiol, 66:3041-3049. The Version of Record is available online at http://dx.doi.org/10.1099/ijsem.0.001143
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Restriction lift date:
2017-08-01
Citation:
Shkoporov, A. N., Chaplin, A. V., Shcherbakova, V. A., Suzina, N. E., Kafarskaia, L. I., Bozhenko, V. K. and Efimov, B. A. (2016) ‘Ruthenibacterium lactatiformans gen. nov., sp. nov., an anaerobic, lactate-producing member of the family Ruminococcaceae isolated from human faeces’, International Journal of Systematic and Evolutionary Microbiology, 66, pp. 3041-3049. doi: 10.1099/ijsem.0.001143
Abstract:
Two novel strains of Gram-stain-negative, rod-shaped, obligately anaerobic, non-spore-forming, non-motile bacteria were isolated from the faeces of healthy human subjects. The strains, designated as 585-1T and 668, were characterized by mesophilic fermentative metabolism, production of d-lactic acid, succinic acid and acetic acid as end products of d-glucose fermentation, prevalence of C18 : 1 ω9, C18 : 1 ω9 aldehyde, C16 : 0 and C16 : 1 ω7c fatty acids, presence of glycine, glutamic acid, lysine, alanine and aspartic acid in the petidoglycan peptide moiety and lack of respiratory quinones. Whole genome sequencing revealed the DNA G+C content was 56.4–56.6 mol%. The complete 16S rRNA gene sequences of the two strains shared 91.7/91.6 % similarity with Anaerofilum pentosovorans FaeT, 91.3/91.2 % with Gemmiger formicilis ATCC 27749T and 88.9/88.8 % with Faecalibacterium prausnitzii ATCC 27768T. On the basis of chemotaxonomic and genomic properties it was concluded that the strains represent a novel species in a new genus within the family Ruminococcaceae , for which the name Ruthenibacterium lactatiformans gen. nov., sp. nov. is proposed. The type strain of Ruthenibacterium lactatiformans is 585-1T (=DSM 100348T=VKM B-2901T).
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