Effect of tempol and tempol plus catalase on intra-renal haemodynamics in spontaneously hypertensive stroke-prone (SHSP) and Wistar rats

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dc.contributor.author Ahmeda, Ahmad F.
dc.contributor.author Rae, Mark G.
dc.contributor.author Al Otaibi, Mohammed F.
dc.contributor.author Anweigi, Lamyia M.
dc.contributor.author Johns, Edward J.
dc.date.accessioned 2017-01-24T11:59:34Z
dc.date.available 2017-01-24T11:59:34Z
dc.date.issued 2016-12-09
dc.identifier.citation Ahmeda, A. F., Rae, M. G., Al Otaibi, M. F., Anweigi, L. M. and Johns, E. J. (2016) 'Effect of tempol and tempol plus catalase on intra-renal haemodynamics in spontaneously hypertensive stroke-prone (SHSP) and Wistar rats', Journal of Physiology and Biochemistry, pp. 1-8. First Online. doi: 10.1007/s13105-016-0541-1 en
dc.identifier.uri http://hdl.handle.net/10468/3508
dc.identifier.doi 10.1007/s13105-016-0541-1
dc.description.abstract Vasoconstriction within the renal medulla contributes to the development of hypertension. This study investigated the role of reactive oxygen species (ROS) in regulating renal medullary and cortical blood perfusion (MBP and CBP respectively) in both stroke-prone spontaneously hypertensive rats (SHRSP) and Wistar rats. CBP and MBP were measured using a laser-Doppler flow meter before and after intra-renal infusion of tempol, the superoxide dismutase (SOD) mimetic or tempol plus catalase, the hydrogen peroxide-degrading enzyme. Tempol infusion significantly elevated blood perfusion within the renal medulla (MBP) in both SHRSP (by 43 ± 7%, P < 0.001) and Wistar rats (by 17 ± 2%, P < 0.05) but the magnitude of the increase was significantly greater in the SHRSP (P < 0.01). When the enzyme catalase and tempol were co-infused, MBP was again significantly increased in SHRSP (by 57 ± 6%, P < 0.001) and Wistar rats (by 33 ± 6%, P < 0.001), with a significantly greater increase in perfusion being induced in the SHRSP relative to the Wistar rats (P < 0.01). Notably, this increase was significantly greater than in those animals infused with tempol alone (P < 0.01). These results suggest that ROS plays a proportionally greater role in reducing renal vascular compliance, particularly within the renal medulla, in normotensive and hypertensive animals, with effects being greater in the hypertensive animals. This supports the hypothesis that SHRSP renal vasculature might be subjected to elevated level of oxidative stress relative to normotensive animals. en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Springer Verlag en
dc.rights © University of Navarra 2016. Published by Springer Verlag. The final publication is available at Springer via http://dx.doi.org/10.1007/s13105-016-0541-1 en
dc.subject Renal blood perfusion en
dc.subject Tempol en
dc.subject Catalase en
dc.subject Reactive oxygen species en
dc.subject SHRSP en
dc.title Effect of tempol and tempol plus catalase on intra-renal haemodynamics in spontaneously hypertensive stroke-prone (SHSP) and Wistar rats en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Mark Rae, Physiology, University College Cork, Cork, Ireland. +353-21-490-3000 Email: m.rae@ucc.ie en
dc.internal.availability Full text available en
dc.check.info Access to this item is restricted until 12 months after publication by the request of the publisher. en
dc.check.date 2017-12-09
dc.date.updated 2017-01-10T13:49:12Z
dc.description.version Accepted Version en
dc.internal.rssid 327904269
dc.contributor.funder College of Medicine, King Saud University en
dc.description.status Peer reviewed en
dc.identifier.journaltitle Journal Of Physiology And Biochemistry en
dc.internal.copyrightchecked No. !!CORA!! en
dc.internal.licenseacceptance Yes en
dc.internal.IRISemailaddress m.rae@ucc.ie en


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