Methanosarcina play an important role in anaerobic co-digestion of the seaweed Ulva lactuca: metagenomics structure and predicted metabolism of functional microbial communities.

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dc.contributor.author FitzGerald, Jamie A.
dc.contributor.author Allen, Eoin
dc.contributor.author Wall, David M.
dc.contributor.author Jackson, Stephen A.
dc.contributor.author Murphy, Jerry D.
dc.contributor.author Dobson, Alan D. W.
dc.date.accessioned 2017-02-02T16:21:45Z
dc.date.available 2017-02-02T16:21:45Z
dc.date.issued 2015-11-10
dc.identifier.citation FitzGerald, J. A., Allen, E., Wall, D. M., Jackson, S. A., Murphy, J. D. and Dobson, A. D. W. (2015) 'Methanosarcina Play an Important Role in Anaerobic Co-Digestion of the Seaweed Ulva lactuca: Taxonomy and Predicted Metabolism of Functional Microbial Communities', PLOS ONE, 10(11), pp. e0142603. doi:10.1371/journal.pone.0142603 en
dc.identifier.volume 10 en
dc.identifier.issued 11 en
dc.identifier.startpage e0142603-1 en
dc.identifier.endpage e0142603-21 en
dc.identifier.issn 1932-6203
dc.identifier.uri http://hdl.handle.net/10468/3557
dc.identifier.doi 10.1371/journal.pone.0142603
dc.description.abstract Macro-algae represent an ideal resource of third generation biofuels, but their use necessitates a refinement of commonly used anaerobic digestion processes. In a previous study, contrasting mixes of dairy slurry and the macro-alga Ulva lactuca were anaerobically digested in mesophilic continuously stirred tank reactors for 40 weeks. Higher proportions of U. lactuca in the feedstock led to inhibited digestion and rapid accumulation of volatile fatty acids, requiring a reduced organic loading rate. In this study, 16S pyrosequencing was employed to characterise the microbial communities of both the weakest (R1) and strongest (R6) performing reactors from the previous work as they developed over a 39 and 27-week period respectively. Comparing the reactor communities revealed clear differences in taxonomy, predicted metabolic orientation and mechanisms of inhibition, while constrained canonical analysis (CCA) showed ammonia and biogas yield to be the strongest factors differentiating the two reactor communities. Significant biomarker taxa and predicted metabolic activities were identified for viable and failing anaerobic digestion of U. lactuca. Acetoclastic methanogens were inhibited early in R1 operation, followed by a gradual decline of hydrogenotrophic methanogens. Near-total loss of methanogens led to an accumulation of acetic acid that reduced performance of R1, while a slow decline in biogas yield in R6 could be attributed to inhibition of acetogenic rather than methanogenic activity. The improved performance of R6 is likely to have been as a result of the large Methanosarcina population, which enabled rapid removal of acetic acid, providing favourable conditions for substrate degradation. en
dc.description.sponsorship Science Foundation Ireland (Grant Numbers (11/ RFP.1/ENM/3213) and (12/RC/2302)); Teagasc (Walsh Fellowship); Marine Instititue (Beaufort Marine Research Award, part of the Sea Change Strategy and the Strategy for Science Technology and Innovation (2006–2012), with the support of The Marine Institute under the Marine Research Sub-Programme of the National Development Plan 2007–2013.) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Public Library of Science en
dc.rights © 2015 FitzGerald et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. en
dc.rights.uri https://creativecommons.org/licenses/by/4.0/ en
dc.subject Biogas en
dc.subject Methanogens en
dc.subject Archaean biology en
dc.subject Acetic acid en
dc.subject Ammonia en
dc.subject Slurries en
dc.subject Linear discriminant analysis en
dc.subject Microbial taxonomy en
dc.title Methanosarcina play an important role in anaerobic co-digestion of the seaweed Ulva lactuca: metagenomics structure and predicted metabolism of functional microbial communities. en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Jeremiah D.G. Murphy, Civil Engineering, University College Cork, Cork, Ireland. +353-21-490-3000 Email: jerry.murphy@ucc.ie en
dc.internal.availability Full text available en
dc.date.updated 2017-02-02T16:14:02Z
dc.description.version Published Version en
dc.internal.rssid 352354717
dc.contributor.funder Science Foundation Ireland en
dc.contributor.funder Teagasc en
dc.contributor.funder Marine Institute en
dc.description.status Peer reviewed en
dc.identifier.journaltitle Plos One en
dc.internal.copyrightchecked No !!CORA!! en
dc.internal.licenseacceptance Yes en
dc.internal.IRISemailaddress jerry.murphy@ucc.ie en


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© 2015 FitzGerald et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Except where otherwise noted, this item's license is described as © 2015 FitzGerald et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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