Genetic and functional characterisation of the lactococcal P335 phage-host interactions

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dc.contributor.author Mahony, Jennifer
dc.contributor.author Oliveira, Joana
dc.contributor.author Collins, Barry
dc.contributor.author Hanemaaijer, Laurens
dc.contributor.author Lugli, Gabriele A.
dc.contributor.author Neve, Horst
dc.contributor.author Ventura, Marco
dc.contributor.author Kouwen, Thijs R.
dc.contributor.author Cambillau, Christian
dc.contributor.author van Sinderen, Douwe
dc.date.accessioned 2017-02-22T12:19:09Z
dc.date.available 2017-02-22T12:19:09Z
dc.date.issued 2017-02-10
dc.identifier.citation Mahony, J., Oliveira, J., Collins, B., Hanemaaijer, L., Lugli, G. A., Neve, H., Ventura, M., Kouwen, T. R., Cambillau, C. and van Sinderen, D. (2017) 'Genetic and functional characterisation of the lactococcal P335 phage-host interactions', BMC Genomics, 18(1), pp. 146. doi:10.1186/s12864-017-3537-5 en
dc.identifier.volume 18 en
dc.identifier.issued 1 en
dc.identifier.startpage 146-1 en
dc.identifier.endpage 146-16 en
dc.identifier.issn 1471-2164
dc.identifier.uri http://hdl.handle.net/10468/3666
dc.identifier.doi 10.1186/s12864-017-3537-5
dc.description.abstract Background: Despite continuous research efforts, bacterio(phages) infecting Lactococcus lactis starter strains persist as a major threat to dairy fermentations. The lactococcal P335 phages, which are currently classified into four sub-groups (I-IV), are the second most frequently isolated phage group in an industrial dairy context. Results: The current work describes the isolation and comparative genomic analysis of 17 novel P335 group phages. Detailed analysis of the genomic region of P335 phages encoding the so-called “baseplate”, which includes the receptor binding protein (RBP) was combined with a functional characterization of the RBP of sub-group III and IV phages. Additionally, calcium-dependence assays revealed a specific requirement for calcium by sub-group IV phages while host range analysis highlighted a higher number of strains with CWPS type A (11 of 39 strains) are infected by the P335 phages assessed in this study than those with a C (five strains), B (three of 39 strains) or unknown (one of 39 strains) CWPS type. Conclusions: These analyses revealed significant divergence among RBP sequences, apparently reflecting their unique interactions with the host and particularly for strains with a type A CWPS. The implications of the genomic architecture of lactococcal P335 phages on serving as a general model for Siphoviridae phages are discussed. en
dc.description.sponsorship Science Foundation Ireland (SFI Technology Innovation Development Award (TIDA) (Ref. No. 14/TIDA/2287), Starting Investigator Research Grant (SIRG) (Ref. No. 15/SIRG/3430), SFI Principal Investigator award (Ref. No. 13/IA/1953)) DSM Food Specialties, the Netherlands. en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher BioMed Central en
dc.rights © The Author(s). 2017 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. en
dc.rights.uri https://creativecommons.org/licenses/by/4.0/ en
dc.subject Bacteriophage en
dc.subject Lactococcus lactis en
dc.subject Dairy Fermentation en
dc.subject Receptor-binding protein en
dc.title Genetic and functional characterisation of the lactococcal P335 phage-host interactions en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Douwe Van Sinderen, Microbiology, University College Cork, Cork, Ireland. +353-21-490-3000 Email: d.vansinderen@ucc.ie en
dc.internal.availability Full text available en
dc.date.updated 2017-02-22T12:02:09Z
dc.description.version Published Version en
dc.internal.rssid 384563552
dc.contributor.funder Science Foundation Ireland en
dc.contributor.funder DSM Food Specialties, the Netherlands.
dc.description.status Peer reviewed en
dc.identifier.journaltitle BMC Genomics en
dc.internal.copyrightchecked No !!CORA!! en
dc.internal.licenseacceptance Yes en
dc.internal.IRISemailaddress d.vansinderen@ucc.ie en


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© The Author(s). 2017 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. Except where otherwise noted, this item's license is described as © The Author(s). 2017 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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