Controlled functional expression of the bacteriocins pediocin PA-1 and bactofencin A in Escherichia coli

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Date
2017-06-08
Authors
Mesa-Pereira, Beatriz
O'Connor, Paula M.
Rea, Mary C.
Cotter, Paul D.
Hill, Colin
Ross, R. Paul
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Nature Publishing Group
Published Version
10.1038/s41598-017-02868-w
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Abstract
The bacteriocins bactofencin A (class IId) and pediocin PA-1 (class IIa) are encoded by operons with a similarly clustered gene organization including a structural peptide, an immunity protein, an ABC transporter and accessory bacteriocin transporter protein. Cloning of these operons in E. coli TunerTM (DE3) on a pETcoco-2 derived vector resulted in successful secretion of both bacteriocins. A corresponding approach, involving the construction of vectors containing different combinations of these genes, revealed that the structural and the transporter genes alone are sufficient to permit heterologous production and secretion in this host. Even though the accessory protein, usually associated with optimal disulfide bond formation, was not required for bacteriocin synthesis, its presence did result in greater pediocin PA-1 production. The simplicity of the system and the fact that the associated bacteriocins could be recovered from the extracellular medium provides an opportunity to facilitate protein engineering and the overproduction of biologically-active bacteriocins at industrial scale. Additionally, this system could enable the characterization of new bacteriocin operons where genetic tools are not available for the native producers.
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Keywords
Bacteriocin operons , Bactofencin A (class IId) , Pediocin PA-1 (class IIa) , Escherichia coli
Citation
Mesa-Pereira, B., O’Connor, P. M., Rea, M. C., Cotter, P. D., Hill, C. and Ross, R. P. (2017) 'Controlled functional expression of the bacteriocins pediocin PA-1 and bactofencin A in Escherichia coli', Scientific Reports, 7(1), 3069. doi:10.1038/s41598-017-02868-w
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https://hdl.handle.net/10468/4189
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Microbiology - Journal Articles
APC Microbiome Ireland - Journal Articles
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© The Authors 2017. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.