Global survey and genome exploration of bacteriophages infecting the lactic acid bacterium Streptococcus thermophilus

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dc.contributor.author McDonnell, Brian
dc.contributor.author Mahony, Jennifer
dc.contributor.author Hanemaaijer, Laurens
dc.contributor.author Neve, Horst
dc.contributor.author Noben, Jean-Paul
dc.contributor.author Lugli, Gabriele A.
dc.contributor.author Ventura, Marco
dc.contributor.author Kouwen, Thijs R.
dc.contributor.author van Sinderen, Douwe
dc.date.accessioned 2017-10-18T09:40:15Z
dc.date.available 2017-10-18T09:40:15Z
dc.date.issued 2017
dc.identifier.citation McDonnell, B., Mahony, J., Hanemaaijer, L., Neve, H., Noben, J.-P., Lugli, G. A., Ventura, M., Kouwen, T. R. and van Sinderen, D. (2017) 'Global survey and genome exploration of bacteriophages infecting the lactic acid bacterium Streptococcus thermophilus', Frontiers in Microbiology, 8, 1754 (15pp). doi: 10.3389/fmicb.2017.01754 en
dc.identifier.volume 8
dc.identifier.startpage 1
dc.identifier.endpage 15
dc.identifier.issn 1664-302X
dc.identifier.uri http://hdl.handle.net/10468/4900
dc.identifier.doi 10.3389/fmicb.2017.01754
dc.description.abstract Despite the persistent and costly problem caused by (bacterio)phage predation of Streptococcus thermophilus in dairy plants, DNA sequence information relating to these phages remains limited. Genome sequencing is necessary to better understand the diversity and proliferative strategies of virulent phages. In this report, whole genome sequences of 40 distinct bacteriophages infecting S. thermophilus were analyzed for general characteristics, genomic structure and novel features. The bacteriophage genomes display a high degree of conservation within defined groupings, particularly across the structural modules. Supporting this observation, four novel members of a recently discovered third group of S. thermophilus phages (termed the 5093 group) were found to be conserved relative to both phage 5093 and to each other. Replication modules of S. thermophilus phages generally fall within two main groups, while such phage genomes typically encode one putative transcriptional regulator. Such features are indicative of widespread functional synteny across genetically distinct phage groups. Phage genomes also display nucleotide divergence between groups, and between individual phages of the same group (within replication modules and at the 3′ end of the lysis module)—through various insertions and/or deletions. A previously described multiplex PCR phage detection system was updated to reflect current knowledge on S. thermophilus phages. Furthermore, the structural protein complement as well as the antireceptor (responsible for the initial attachment of the phage to the host cell) of a representative of the 5093 group was defined. Our data more than triples the currently available genomic information on S. thermophilus phages, being of significant value to the dairy industry, where genetic knowledge of lytic phages is crucial for phage detection and monitoring purposes. In particular, the updated PCR detection methodology for S. thermophilus phages is highly useful in monitoring particular phage group(s) present in a given whey sample. Studies of this nature therefore not only provide information on the prevalence and associated threat of known S. thermophilus phages, but may also uncover newly emerging and genomically distinct phages infecting this dairy starter bacterium. en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Frontiers Media en
dc.relation.uri https://www.frontiersin.org/articles/10.3389/fmicb.2017.01754/full
dc.rights © 2017, McDonnell, Mahony, Hanemaaijer, Neve, Noben, Lugli, Ventura, Kouwen and van Sinderen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. en
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject Panvirome en
dc.subject Methyltransferase en
dc.subject Transcriptional regulator en
dc.subject Structural proteome en
dc.subject Antireceptor en
dc.title Global survey and genome exploration of bacteriophages infecting the lactic acid bacterium Streptococcus thermophilus en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Douwe van Sinderen, Microbiology, University College Cork, Cork, Ireland. +353-21-490-3000. Email: d.vansinderen@ucc.ie en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.contributor.funder Science Foundation Ireland
dc.contributor.funder Royal DSM
dc.description.status Peer reviewed en
dc.identifier.journaltitle Frontiers in Microbiology en
dc.internal.IRISemailaddress d.vansinderen@ucc.ie en
dc.identifier.articleid 1754
dc.relation.project info:eu-repo/grantAgreement/SFI/SFI Starting Investigator Research Grant (SIRG)/15/SIRG/3430/IE/Phage-host interactome of the dairy bacterium Streptococcus thermophilus (PHIST)/
dc.relation.project info:eu-repo/grantAgreement/SFI/SFI Investigator Programme/13/IA/1953/IE/Functional analysis of the host adsorption and DNA injection processes of a lactococcal bacteriophage/


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© 2017, McDonnell, Mahony, Hanemaaijer, Neve, Noben, Lugli, Ventura, Kouwen and van Sinderen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. Except where otherwise noted, this item's license is described as © 2017, McDonnell, Mahony, Hanemaaijer, Neve, Noben, Lugli, Ventura, Kouwen and van Sinderen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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