Monitoring denaturation behaviour and comparative stability of DNA triple helices using oligonucleotide–gold nanoparticle conjugates
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Date
2004
Authors
Murphy, Deirdre
Eritja, Ramon
Redmond, Gareth
Journal Title
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Publisher
Oxford University Press
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Abstract
Gold nanoparticle labels, combined with UV-visible optical absorption spectroscopic methods, are employed to probe the temperature-dependent solution properties of DNA triple helices. By using oligonucleotide-nanoparticle conjugates to characterize triplex denaturation, for the first time triplex to duplex melting transitions may be sensitively monitored, with minimal signal interference from duplex to single strand melting, for both parallel and antiparallel triple helices. Further, the comparative sequence-dependent stability of DNA triple helices may also be examined using this approach. Specifically, triplex to duplex melting transitions for triplexes formed using oligonucleotides that incorporate 8-aminoguanine derivatives were successfully monitored and stabilization of both parallel and antiparallel triplexes following 8-aminoguanine substitutions is demonstrated.
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Keywords
3rd strand , Colorimetric detection , Purine , 8-Amino-2'-deoxyguanosine , Polynucleotides , Stabilization , Parallel , Duplex
Citation
Murphy, D., Eritja, R. and Redmond, G. (2004) 'Monitoring denaturation behaviour and comparative stability of DNA triple helices using oligonucleotide–gold nanoparticle conjugates', Nucleic Acids Research, 32(7), e65 (7pp). doi: 10.1093/nar/gnh065
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© 2004, Oxford University Press all rights reserved