Monitoring denaturation behaviour and comparative stability of DNA triple helices using oligonucleotide–gold nanoparticle conjugates

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dc.contributor.author Murphy, Deirdre
dc.contributor.author Eritja, Ramon
dc.contributor.author Redmond, Gareth
dc.date.accessioned 2017-11-14T13:24:32Z
dc.date.available 2017-11-14T13:24:32Z
dc.date.issued 2004
dc.identifier.citation Murphy, D., Eritja, R. and Redmond, G. (2004) 'Monitoring denaturation behaviour and comparative stability of DNA triple helices using oligonucleotide–gold nanoparticle conjugates', Nucleic Acids Research, 32(7), e65 (7pp). doi: 10.1093/nar/gnh065 en
dc.identifier.volume 32
dc.identifier.issued 7
dc.identifier.issn 0305-1048
dc.identifier.uri http://hdl.handle.net/10468/5034
dc.identifier.doi 10.1093/nar/gnh065
dc.description.abstract Gold nanoparticle labels, combined with UV-visible optical absorption spectroscopic methods, are employed to probe the temperature-dependent solution properties of DNA triple helices. By using oligonucleotide-nanoparticle conjugates to characterize triplex denaturation, for the first time triplex to duplex melting transitions may be sensitively monitored, with minimal signal interference from duplex to single strand melting, for both parallel and antiparallel triple helices. Further, the comparative sequence-dependent stability of DNA triple helices may also be examined using this approach. Specifically, triplex to duplex melting transitions for triplexes formed using oligonucleotides that incorporate 8-aminoguanine derivatives were successfully monitored and stabilization of both parallel and antiparallel triplexes following 8-aminoguanine substitutions is demonstrated. en
dc.description.sponsorship Higher Education Authority (PRTLI1 Nanoscale Science and Technology Initiative); Dirección General de Investigación Científica y Técnica (BQU2000-0649 and BQU2003-00397); Generalitat de Catalunya (2001-SGR-0049)
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Oxford University Press en
dc.relation.uri https://academic.oup.com/nar/article-lookup/doi/10.1093/nar/gnh065
dc.rights © 2004, Oxford University Press all rights reserved en
dc.subject 3rd strand en
dc.subject Colorimetric detection en
dc.subject Purine en
dc.subject 8-Amino-2'-deoxyguanosine en
dc.subject Polynucleotides en
dc.subject Stabilization en
dc.subject Parallel en
dc.subject Duplex en
dc.title Monitoring denaturation behaviour and comparative stability of DNA triple helices using oligonucleotide–gold nanoparticle conjugates en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Redmond, G (reprint author), NMRC, Nanotechnol Grp, Cork, Ireland. en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.contributor.funder Sixth Framework Programme
dc.contributor.funder Higher Education Authority
dc.contributor.funder Dirección General de Investigación Científica y Técnica
dc.contributor.funder Generalitat de Catalunya
dc.description.status Peer reviewed en
dc.identifier.journaltitle Nucleic Acids Research en
dc.internal.IRISemailaddress gareth.redmond@nmrc.ie en
dc.identifier.articleid e65
dc.relation.project info:eu-repo/grantAgreement/NIH/NATIONAL CENTER FOR RESEARCH RESOURCES/2M01RR001066-21/US/SFTY &EFFCY OF SUBSTANCE-P ANTAGONIST CJ-11974 &PLBO IN SUBJ W/ULCER COLITIS/


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