Triangular gold nanoparticles modify shell characteristics and increase antioxidant enzyme activities in the clam Ruditapes decussatus
Abdelhafidh, Khazri; Badreddine, Sellami; Mezni, Amine; Mouhamed, Dellali; Wiem, Saidani; Imen, Bouzidi; Sheehan, David; Mahmoudi, Ezzeddine; Hamouda, Beyrem
Date:
2018-04-07
Copyright:
© 2018 Informa UK Limited, trading as Taylor & Francis Group. This is an Accepted Manuscript of an article published by Taylor & Francis in Biomarkers on 19 April 2018, available online: http://www.tandfonline.com/10.1080/1354750X.2018.1463565
Full text restriction information:
Access to this article is restricted until 12 months after publication by the request of the publisher.
Restriction lift date:
2019-04-19
Citation:
Abdelhafidh, K., Badreddine, S., Mezni, A., Mouhamed, D., Wiem, S., Imen, B., David, S., Mahmoudi, E. and Hamouda, B. (2018) 'Triangular gold nanoparticles modify shell characteristics and increase antioxidant enzyme activities in the clam Ruditapes decussatus', Biomarkers, In Press, doi: 10.1080/1354750X.2018.1463565
Abstract:
Context: Nanoparticles may cause adverse environmental effects but there is limited information on their interactions with marine organisms. Objective: Our aim was to examine the effects of triangular gold nanoparticles (Tr-Au NPs) on the clam, Ruditapes decussatus. Materials and methods: Clams were exposed to Tr-Au1 = 5 µg/L and Tr-Au2 = 10 µg/L for 2 and 7 days. Effects on shell structure were investigated. Superoxide dismutase (SOD), catalase (CAT), glutathione transferase (GST) activities, protein carbonyl levels and malondialdehyde content were used to assess biochemical status. Results: Transmission electron microscopy (TEM) and electron dispersive X-ray microanalysis (EDX) showed that Tr-Au NPs modified shell structure and morphology. Tr-Au NPs size increased forming aggregate particles. Tr-Au NPs increased SOD, CAT and GST activities in gill and digestive gland in a concentration- and time-dependent manner indicating defence against oxidative stress. Enhanced lipid peroxidation and protein carbonyl levels confirmed oxidative stress. Conclusion: Tr-Au NPs cause oxidative stress and affect shell structure of clams. These findings may have relevance to other marine species.
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