Access to this article is restricted until 12 months after publication by request of the publisher.. Restriction lift date: 2019-07-03
HPLC-ESI-MS/MS characterisation of metabolites produced by Pseudovibrio sp. W64, a marine sponge-derived bacterium isolated from Irish waters
Loading...
Files
Figure S1 and Figure S48
Date
2018-07-03
Authors
Choudhary, Alka
Naughton, Lynn M.
Dobson, Alan D. W.
Rai, Dilip K.
Journal Title
Journal ISSN
Volume Title
Publisher
John Wiley & Sons, Inc.
Published Version
Abstract
Rationale: In recent years, metabolites produced by Pseudovibrio species have gained scientific attention due to their potent antimicrobial activity. Recently we also have assessed antibacterial activities of Pseudovibrio sp. W64 isolates against Staphylococcus aureus, where only the dominant tropodithietic acid (TDA) was identified. However characterisation of other metabolites is necessary as these metabolites may also serve as potent antimicrobial agents. Methods: LC-MS/MS, aided by accurate mass measurements, was employed to screen and characterise a range of metabolites produced by Pseudovibrio sp. W64 via assessment of ethyl acetate fractions generated from bacterial cultures. Results: Thirteen metabolites unique to the bacterial culture were detected and their chemical structures were assigned by tandem mass spectrometry and accurate mass measurement. Among the thirteen metabolites, a methyl ester of TDA, a number of cholic acid derivatives, and amino diols and triols were characterised. Conclusions: Pseudovibrio sp. W64 produces methylated TDA in addition to TDA, and metabolises lipids and amino acids in the cell-culture medium. To the best of our knowledge, this is the first report of methylated TDA, cholic acid and its various analogs, and sphinganine being detected in this Pseudovibrio strain. The data generated may help to better understand the biochemical processes and metabolism of bacterial strains towards discovery of antimicrobial agents from marine sources.
Description
Keywords
Pseudovibrio sp. , Tropodithietic acid , Cholic acid , Sphinganine , HPLC-MS/MS
Citation
Choudhary, A., Naughton, L. M., Dobson, A. D.W. and Rai, D. K. (2018) 'HPLC-ESI-MS/MS characterisation of metabolites produced by Pseudovibrio sp. W64, a marine sponge-derived bacterium isolated from Irish waters', Rapid Communications in Mass Spectrometry. doi:10.1002/rcm.8226
Link to publisher’s version
Copyright
© 2018, John Wiley & Sons Inc. This is the peer reviewed version of the following article: Choudhary, A., Naughton, L. M., Dobson, A. D.W. and Rai, D. K. (2018) 'HPLC-ESI-MS/MS characterisation of metabolites produced by Pseudovibrio sp. W64, a marine sponge-derived bacterium isolated from Irish waters', Rapid Communications in Mass Spectrometry. doi:10.1002/rcm.8226, which has been published in final form at https://doi.org/10.1002/rcm.8226. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.