Removal of Listeria monocytogenes dual-species biofilms using combined enzyme-benzalkonium chloride treatments

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dc.contributor.author Rodriguez-Lopez, Pedro
dc.contributor.author Carballo-Justo, Alba
dc.contributor.author Draper, Lorraine A.
dc.contributor.author Cabo, Marta L.
dc.date.accessioned 2018-07-30T10:30:33Z
dc.date.available 2018-07-30T10:30:33Z
dc.date.issued 2017
dc.identifier.volume 33
dc.identifier.issued 1
dc.identifier.startpage 45
dc.identifier.endpage 58
dc.identifier.issn 0892-7014
dc.identifier.uri http://hdl.handle.net/10468/6525
dc.identifier.doi 10.1080/08927014.2016.1261847
dc.description.abstract The effects of pronase (PRN), cellulase (CEL) or DNaseI alone or combined with benzalkonium chloride (BAC) against Listeria monocytogenes-carrying biofilms were assayed. The best removal activity against L. monocytogenes-Escherichia coli biofilms was obtained using DNaseI followed by PRN and CEL. Subsequently, a modified logistic model was used to quantify the combined effects of PRN or DNaseI with BAC. A better BAC performance after PRN compared to DNaseI eradicating L. monocytogenes was observed. In E. coli the effects were the opposite. Finally, effects of DNaseI and DNaseI-BAC treatments were compared against two different L. monocytogenes-carrying biofilms. DNaseI-BAC was more effective against L. monocytogenes when co-cultured with E. coli. Nonetheless, comparing the removal effects after BAC addition, these were higher in mixed-biofilms with Pseudomonas fluorescens. However, a high number of released viable cells was observed after combined treatments. These results open new perspectives of enzymes as an anti-biofilm strategy for environmental pathogen control. en
dc.description.sponsorship Spanish Ministerio de Economia y Competitividad (ENZYMONO) (AGL2010-22212-C02-02); FPI programme (BES-2011-050544) en
dc.description.sponsorship Ministerio de Economía y Competitividad (ENZYMONO, AGL2010-22212-C02-02). European Commission (FPI programme Grant number: BES-2011- 050544)
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Taylor & Francis Ltd en
dc.rights © 2016, Taylor & Francis Group. This is an Accepted Manuscript of an article published by Taylor & Francis in Biofouling on 5th December, 2016, available online: https://www.tandfonline.com/10.1080/08927014.2016.1261847 en
dc.subject Benzalkonium chloride en
dc.subject Biofilm en
dc.subject Disinfection en
dc.subject Enzymes en
dc.subject Fluorescence microscopy en
dc.subject Listeria monocytogenes en
dc.title Removal of Listeria monocytogenes dual-species biofilms using combined enzyme-benzalkonium chloride treatments en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Lorraine Draper, APC Microbiome Institute, University College Cork, Cork, Ireland. T: +353-21-490-3000 E: l.draper@ucc.ie
dc.internal.availability Full text available en
dc.description.version Accepted Version en
dc.contributor.funder Ministerio de Economía y Competitividad
dc.contributor.funder European Commission
dc.description.status Peer reviewed en
dc.identifier.journaltitle Biofouling en


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