Removal of Listeria monocytogenes dual-species biofilms using combined enzyme-benzalkonium chloride treatments
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Accepted Version
Date
2017
Authors
Rodriguez-Lopez, Pedro
Carballo-Justo, Alba
Draper, Lorraine A.
Cabo, Marta L.
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Taylor & Francis Ltd
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Abstract
The effects of pronase (PRN), cellulase (CEL) or DNaseI alone or combined with benzalkonium chloride (BAC) against Listeria monocytogenes-carrying biofilms were assayed. The best removal activity against L. monocytogenes-Escherichia coli biofilms was obtained using DNaseI followed by PRN and CEL. Subsequently, a modified logistic model was used to quantify the combined effects of PRN or DNaseI with BAC. A better BAC performance after PRN compared to DNaseI eradicating L. monocytogenes was observed. In E. coli the effects were the opposite. Finally, effects of DNaseI and DNaseI-BAC treatments were compared against two different L. monocytogenes-carrying biofilms. DNaseI-BAC was more effective against L. monocytogenes when co-cultured with E. coli. Nonetheless, comparing the removal effects after BAC addition, these were higher in mixed-biofilms with Pseudomonas fluorescens. However, a high number of released viable cells was observed after combined treatments. These results open new perspectives of enzymes as an anti-biofilm strategy for environmental pathogen control.
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Keywords
Benzalkonium chloride , Biofilm , Disinfection , Enzymes , Fluorescence microscopy , Listeria monocytogenes
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© 2016, Taylor & Francis Group. This is an Accepted Manuscript of an article published by Taylor & Francis in Biofouling on 5th December, 2016, available online: https://www.tandfonline.com/10.1080/08927014.2016.1261847