Plantaricyclin A, a novel circular bacteriocin produced by Lactobacillus plantarum NI326: purification, characterization, and heterologous production

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dc.contributor.author Borrero, Juan
dc.contributor.author Kelly, Eoin
dc.contributor.author O'Connor, Paula M.
dc.contributor.author Kelleher, Philip
dc.contributor.author Scully, Colm
dc.contributor.author Cotter, Paul D.
dc.contributor.author Mahony, Jennifer
dc.contributor.author van Sinderen, Douwe
dc.date.accessioned 2018-11-22T12:44:42Z
dc.date.available 2018-11-22T12:44:42Z
dc.date.issued 2017-12-15
dc.identifier.citation Borrero, J., Kelly, E., O'Connor, P. M., Kelleher, P., Scully, C., Cotter, P. D., Mahony, J. and van Sinderen, D. (2017) 'Plantaricyclin A, a novel circular bacteriocin produced by Lactobacillus plantarum NI326: purification, characterization, and heterologous production', Applied and Environmental Microbiology, 84(1), e01801-17 (10pp). doi:10.1128/aem.01801-17 en
dc.identifier.volume 84 en
dc.identifier.issued 1 en
dc.identifier.issn 0099-2240
dc.identifier.issn 1098-5336
dc.identifier.uri http://hdl.handle.net/10468/7134
dc.identifier.doi 10.1128/aem.01801-17
dc.description.abstract Bacteriocins from lactic acid bacteria (LAB) are of increasing interest in recent years due to their potential as natural preservatives against food and beverage spoilage microorganisms. In a screening study for LAB, we isolated from olives a strain, Lactobacillus plantarum NI326, with activity against the beverage-spoilage bacterium Alicyclobacillus acidoterrestris. Genome sequencing of NI326 enabled the identification of a gene cluster (designated plc) encoding a putative circular bacteriocin and proteins involved in its modification, transport, and immunity. This novel bacteriocin, named plantaricyclin A (PlcA), was grouped into the circular bacteriocin subgroup II due to its high degree of similarity with other gassericin A-like bacteriocins. Purification of PlcA from the supernatant of Lb. plantarum NI326 resulted in an active peptide with a molecular mass of 5,570 Da, corresponding to that predicted from the (processed) PlcA amino acid sequence. The plc gene cluster was cloned and expressed in Lactococcus lactis NZ9000, resulting in the production of an active 5,570-Da bacteriocin in the supernatant. PlcA is believed to be produced as a 91-amino-acid precursor with a 33-amino-acid leader peptide, which is predicted to be removed, followed by joining of the N and C termini via a covalent linkage to form the mature 58-amino-acid circular bacteriocin PlcA. We report the characterization of a circular bacteriocin produced by Lb. plantarum. The inhibition displayed against A. acidoterrestris highlights its potential use as a preservative in food and beverages.IMPORTANCE In this work, we describe the purification and characterization of an antimicrobial peptide, termed plantaricyclin A (PlcA), produced by a Lactobacillus plantarum strain isolated from olives. This peptide has a circular structure, and all genes involved in its production, circularization, and secretion were identified. PlcA shows antimicrobial activity against different strains, including Alicyclobacillus acidoterrestris, a common spoilage bacterium, which causes substantial economic losses in the beverage industry every year. In this study, we describe a circular antimicrobial peptide, PlcA, for a Lactobacillus plantarum strain. en
dc.description.sponsorship Enterprise Ireland (Innovation Partnership Programme IP/2013/0254) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher American Society for Microbiology en
dc.rights © 2017, American Society for Microbiology. All Rights Reserved. en
dc.subject Circular bacteriocin en
dc.subject Alicyclobacillus acidoterrestris en
dc.subject Lactobacillus plantarum en
dc.subject Immunity en
dc.title Plantaricyclin A, a novel circular bacteriocin produced by Lactobacillus plantarum NI326: purification, characterization, and heterologous production en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Douwe Van Sinderen, Microbiology, University College Cork, Cork, Ireland. +353-21-490-3000 Email: d.vansinderen@ucc.ie en
dc.internal.availability Full text available en
dc.date.updated 2018-11-22T12:34:50Z
dc.description.version Accepted Version en
dc.internal.rssid 462822839
dc.contributor.funder Enterprise Ireland en
dc.contributor.funder Science Foundation Ireland en
dc.description.status Peer reviewed en
dc.identifier.journaltitle Applied and Environmental Microbiology en
dc.internal.copyrightchecked Yes en
dc.internal.licenseacceptance Yes en
dc.internal.IRISemailaddress d.vansinderen@ucc.ie en
dc.identifier.articleid e01801-17
dc.relation.project info:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2273/IE/Alimentary Pharmabiotic Centre (APC) - Interfacing Food & Medicine/ en
dc.relation.project info:eu-repo/grantAgreement/SFI/SFI Starting Investigator Research Grant (SIRG)/15/SIRG/3430/IE/Phage-host interactome of the dairy bacterium Streptococcus thermophilus (PHIST)/ en


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