Bifidobacterium animalis AHC7 protects against pathogen-induced NF-ҝB activation in vivo
Loading...
Files
Published Version
Date
2010-12-22
Authors
O'Mahony, David
Murphy, Sharon
Boileau, Thomas
Park, JeanSoon
O'Brien, Frances
Groeger, David S.
Konieczna, Patrycja
Ziegler, Mario
Scully, Paul
Shanahan, Fergus
Journal Title
Journal ISSN
Volume Title
Publisher
BioMed Central
Published Version
Abstract
Background: Bifidobacteria and lactobacilli are among the early and important colonizers of the gastrointestinal
tract and are generally considered to be part of a normal, healthy microbiota. It is believed that specific strains
within the microbiota can influence host immune-reactivity and may play a role in protection from infection and
aberrant inflammatory activity. One such strain, Bifidobacterium animalis AHC7, has been previously shown to
protect against Salmonella typhimurium infection in mice and helps resolve acute idiopathic diarrhea in dogs. The
aim of this study was to investigate the potential molecular and cellular mechanisms underpinning the
Bifidobacterium animalis AHC7 protective effect.
Results: Following 4 hours of infection with Salmonella typhimurium, NF-ҝB activation was significantly elevated in
vivo in placebo and Enterococcus faecium-fed animals while Bifidobacterium animalis AHC7 consumption significantly attenuated the NF- B response. In vitro anti-CD3/CD28 stimulated Peyer’s patch cells secreted significantly less TNF-a and IFN-g following Bifidobacterium animalis AHC7 consumption. Stimulated cells released more IL-12p70 but this difference did not reach statistical significance. No alteration in mucosal IL-6, IL-10 or MCP-1 levels were observed. No statistically significant change in the cytokine profile of mesenteric lymph node cells was noted. In vitro, Bifidobacterium animalis AHC7 was bound by dendritic cells and induced secretion of both IL-10 and IL-12p70. In addition, co-culture of CD4+ T cells with Bifidobacterium animalis AHC7-stimulated dendritic cells
resulted in a significant increase in CD25+Foxp3+ T cell numbers.
Conclusion: Bifidobacterium animalis AHC7 exerts an anti-inflammatory effect via the attenuation of proinflammatory
transcription factor activation in response to an infectious insult associated with modulation of proinflammatory
cytokine production within the mucosa. The cellular mechanism underpinning Bifidobacterium
animalis AHC7 mediated attenuation of NF- B activation may include recognition of the bacterium by dendritic
cells and induction of CD25+Foxp3+ T cells.
Description
Keywords
Bifidobacterium animalis AHC7 , Salmonella typhimurium , NF-ҝB , Idiopathic Diarrhea , in vivo , Anti-inflammatory Effect
Citation
O'Mahony D, Murphy S, Boileau T, Park JS, O'Brien F, Groeger D, Konieczna P, Ziegler M, Scully P, Shanahan F, Kiely B, O'Mahony L. Bifidobacterium animalis AHC7 protects against pathogen-induced NF-κB activation in vivo. (2010) BMC Immunology. 11:63. doi: 10.1186/1471-2172-11-63