Optimization and Validation of an HPAEC-PAD Method for the Quantification of FODMAPs in Cereals and Cereal-Based Products

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Ispiryan, Lilit
Heitmann, Mareile
Hoehnel, Andrea
Zannini, Emanuele
Arendt, Elke K.
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American Chemical Society, ACS
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This study presents an analytical method for the quantification of fermentable oligo-, di-, and monosaccharides and polyols (FODMAPs) in cereals and cereal-based products, considering diverse ingredients, such as different cereals in addition to wheat, pulses, or pseudocereals. All carbohydrates have been separated, identified, and quantified with a high-performance anion-exchange chromatographic system coupled with a pulsed amperometric detection (HPAEC-PAD). The total fructan content and the average degree of polymerization (DPav) have been determined after enzymatic hydrolysis to the monomers glucose and fructose, on the basis of the principle of the official method for fructan quantification in food products, AOAC 997.08. The methods for extraction, separation, and detection as well as fructan determination are based on several other studies and were modified in order to minimize interferences in the analysis. The method has been validated with regard to the limits of detection and quantification, the linearity, the repeatability, and the accuracy as well as the DPav of the fructans.
FODMAPs , IBS , Cereals , HPAEC-PAD , Fructans , Enzymatic hydrolysis , Quantification , Degree of polymerization , Galactooligosaccharides
Ispiryan, L., Heitmann, M., Hoehnel, A., Zannini, E. and Arendt, E. K. (2019) 'Optimization and Validation of an HPAEC-PAD Method for the Quantification of FODMAPs in Cereals and Cereal-Based Products', Journal of Agricultural and Food Chemistry, 67(15), pp. 4384-4392. doi: 10.1021/acs.jafc.9b00382
© 2019 American Chemical Society. This document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of Agricultural and Food Chemistry, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://pubs.acs.org/doi/10.1021/acs.jafc.9b00382