Identification of dual receptor binding protein systems in Lactococcal 936 group phages

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Date
2018
Authors
Hayes, Stephen
Duhoo, Yoan
Neve, Horst
Murphy, James
Noben, Jean-Paul
Franz, Charles M. A. P.
Cambillau, Christian
Mahony, Jennifer
Nauta, Arjen
van Sinderen, Douwe
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MDPI
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Abstract
Siphoviridae of the lactococcal 936 group are the most commonly encountered bacteriophages in the dairy processing environment. The 936 group phages possess a discrete baseplate at the tip of their tail—a complex harbouring the Receptor Binding Protein (RBP) which is responsible for host recognition and attachment. The baseplate-encoding region is highly conserved amongst 936 phages, with 112 of 115 publicly available phages exhibiting complete synteny. Here, we detail the three exceptions (Phi4.2, Phi4R15L, and Phi4R16L), which differ from this genomic architecture in possessing an apparent second RBP-encoding gene upstream of the “classical” rbp gene. The newly identified RBP possesses an elongated neck region relative to currently defined 936 phage RBPs and is genetically distinct from defined 936 group RBPs. Through detailed characterisation of the representative phage Phi4.2 using a wide range of complementary techniques, we demonstrated that the above-mentioned three phages possess a complex and atypical baseplate structure. Furthermore, the presence of both RBPs in the tail tip of the mature virion was confirmed, while the anticipated host-binding capabilities of both proteins were also verified.
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Virus , Lactic acid bacteria , Structure , Host interactions
Citation
Hayes, S., Duhoo, Y., Neve, H., Murphy, J., Noben, J.P., Franz, C., Cambillau, C., Mahony, J., Nauta, A. and van Sinderen, D., 2018. Identification of Dual Receptor Binding Protein Systems in Lactococcal 936 Group Phages. Viruses, 10(12): 668 (17pp) DOI: 10.3390/v10120668