Human in situ study of the effect of bis(2-methacryloyloxyethyl) dimethylammonium bromide immobilized in dental composite on controlling mature cariogenic biofilm

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dc.contributor.author Melo, Mary Anne S.
dc.contributor.author Weir, Michael D.
dc.contributor.author Passos, Vanara F.
dc.contributor.author Rolim, Juliana P. M.
dc.contributor.author Lynch, Christopher D.
dc.contributor.author Rodrigues, Lidiany K. A.
dc.contributor.author Xu, Hockin H. K.
dc.date.accessioned 2019-08-07T09:29:29Z
dc.date.available 2019-08-07T09:29:29Z
dc.date.issued 2018-11-02
dc.identifier.citation Melo, M., Weir, M., Passos, V., Rolim, J., Lynch, C., Rodrigues, L. and Xu, H., 2018. Human In Situ Study of the effect of Bis (2-Methacryloyloxyethyl) Dimethylammonium Bromide Immobilized in Dental Composite on Controlling Mature Cariogenic Biofilm. International journal of molecular sciences, 19(11):3443 (14pp.). DOI:10.3390/ijms19113443 en
dc.identifier.volume 19 en
dc.identifier.issued 11 en
dc.identifier.startpage 1 en
dc.identifier.endpage 14 en
dc.identifier.issn 1661-6596
dc.identifier.uri http://hdl.handle.net/10468/8289
dc.identifier.doi 10.3390/ijms19113443 en
dc.description.abstract Cariogenic oral biofilms cause recurrent dental caries around composite restorations, resulting in unprosperous oral health and expensive restorative treatment. Quaternary ammonium monomers that can be copolymerized with dental resin systems have been explored for the modulation of dental plaque biofilm growth over dental composite surfaces. Here, for the first time, we investigated the effect of bis(2-methacryloyloxyethyl) dimethylammonium bromide (QADM) on human overlying mature oral biofilms grown intra-orally in human participants for 7–14 days. Seventeen volunteers wore palatal devices containing composite specimens containing 10% by mass of QADM or a control composite without QADM. After 7 and 14 days, the adherent biofilms were collected to determine bacterial counts via colony-forming unit (CFU) counts. Biofilm viability, chronological changes, and percentage coverage were also determined through live/dead staining. QADM composites caused a significant inhibition of Streptococcus mutans biofilm formation for up to seven days. No difference in the CFU values were found for the 14-day period. Our findings suggest that: (1) QADM composites were successful in inhibiting 1–3-day biofilms in the oral environment in vivo; (2) QADM significantly reduced the portion of the S. mutans group; and (3) stronger antibiofilm activity is required for the control of mature long-term cariogenic biofilms. Contact-killing strategies using dental materials aimed at preventing or at least reducing high numbers of cariogenic bacteria seem to be a promising approach in patients at high risk of the recurrence of dental caries around composites. en
dc.description.sponsorship CNPq (141791/2010-1); CAPES (BEX 0574/06-6); University of Maryland (Baltimore Seed grant, University of Maryland School of Dentistry bridge fund) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher MDPI AG en
dc.relation.uri https://www.mdpi.com/1422-0067/19/11/3443
dc.rights © 2018 The Author(s). Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). en
dc.rights.uri https://creativecommons.org/licenses/by/4.0/ en
dc.subject Antibacterial en
dc.subject Biofilm en
dc.subject Caries en
dc.subject Dental composite en
dc.subject Quaternary ammonium monomers en
dc.subject Human in situ study en
dc.title Human in situ study of the effect of bis(2-methacryloyloxyethyl) dimethylammonium bromide immobilized in dental composite on controlling mature cariogenic biofilm en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Chrisopher Lynch, University Dental School and Hospital, University College Cork, Cork, Ireland. +353-21-490-3000 Email: chris.lynch@ucc.ie en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.contributor.funder Conselho Nacional de Desenvolvimento Científico e Tecnológico en
dc.contributor.funder Coordenação de Aperfeiçoamento de Pessoal de Nível Superior en
dc.contributor.funder University of Maryland, Baltimore County en
dc.contributor.funder University of Maryland en
dc.description.status Peer reviewed en
dc.identifier.journaltitle International Journal of Molecular Sciences en
dc.internal.IRISemailaddress chris.lynch@ucc.ie en
dc.identifier.articleid 3443 en
dc.identifier.eissn 1422-0067


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©  2018 The Author(s). Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). Except where otherwise noted, this item's license is described as © 2018 The Author(s). Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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