Dietary a-lactalbumin alters energy balance, gut microbiota composition and intestinal nutrient transporter expression in high-fat diet-fed mice
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Date
2019-05-28
Authors
Boscaini, Serena
Cabrera-Rubio, Raul
Speakman, John R.
Cotter, Paul D.
Cryan, John F.
Nilaweera, Kanishka N.
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Publisher
Cambridge University Press (CUP)
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Abstract
Recently there has been a considerable rise in the frequency of metabolic diseases, such as obesity, due to changes in lifestyle and resultant imbalances between energy intake and expenditure. Whey proteins are considered as potentially important components of a dietary solution to the obesity problem. However, the roles of individual whey proteins in energy balance remain poorly understood. This study investigated the effects of a high-fat diet (HFD) containing α-lactalbumin (LAB), a specific whey protein, or the non-whey protein casein (CAS), on energy balance, nutrient transporters expression and enteric microbial populations. C57BL/6J mice (n 8) were given an HFD containing either 20 % CAS or LAB as protein sources or a low-fat diet containing CAS for 10 weeks. HFD-LAB-fed mice showed a significant increase in cumulative energy intake (P=0·043), without differences in body weight, energy expenditure, locomotor activity, RER or subcutaneous and epididymal white adipose tissue weight. HFD-LAB intake led to a decrease in the expression of glut2 in the ileum (P=0·05) and in the fatty acid transporter cd36 (P<0·001) in both ileum and jejunum. This suggests a reduction in absorption efficiency within the small intestine in the HFD-LAB group. DNA from faecal samples was used for 16S rRNA-based assessment of intestinal microbiota populations; the genera Lactobacillus, Parabacteroides and Bifidobacterium were present in significantly higher proportions in the HFD-LAB group. These data indicate a possible functional relationship between gut microbiota, intestinal nutrient transporters and energy balance, with no impact on weight gain.
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Keywords
Dietary a-lactalbumin , Energy balance , Gut microbiota , Nutrient transporter expression , Whey proteins , High-fat diet , Casein , Cluster differentiation 36 , Epididymal white adipose tissue , Fatty acid synthase , Fatty acid transporter protein 4 , a-lactalbumin , Lactoferrin , Low-fat diet , Neuropeptide Y , Proopiomelanocortin , Subcutaneous white adipose tissue , Whey protein isolate
Citation
Boscaini, S., Cabrera-Rubio, R., Speakman, J. R., Cotter, P. D., Cryan, J. F. and Nilaweera, K. N. (2019) 'Dietary α-lactalbumin alters energy balance, gut microbiota composition and intestinal nutrient transporter expression in high-fat diet-fed mice', British Journal of Nutrition, 121(10), pp. 1097-1107. doi: 10.1017/S0007114519000461
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© The Authors 2019. Published by Cambridge University Press on behalf of the Nutrition Society.