Reconstruction of the bifidobacterial pan-secretome reveals the network of extracellular interactions between bifidobacteria and the infant gut

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dc.contributor.author Lugli, Gabriele A.
dc.contributor.author Mancino, Walter
dc.contributor.author Milani, Christian
dc.contributor.author Duranti, Sabrina
dc.contributor.author Turroni, Francesca
dc.contributor.author van Sinderen, Douwe
dc.contributor.author Ventura, Marco
dc.date.accessioned 2019-08-26T13:52:10Z
dc.date.available 2019-08-26T13:52:10Z
dc.date.issued 2018-06-08
dc.identifier.citation Lugli, G. A., Mancino, W., Milani, C., Duranti, S., Turroni, F., van Sinderen, D. and Ventura, M. (2018) 'Reconstruction of the Bifidobacterial Pan-Secretome Reveals the Network of Extracellular Interactions between Bifidobacteria and the Infant Gut', Applied and Environmental Microbiology, 84(16), e00796-18 (12 pp). doi: 10.1128/aem.00796-18 en
dc.identifier.volume 84 en
dc.identifier.issued 16 en
dc.identifier.startpage 1 en
dc.identifier.endpage 12 en
dc.identifier.issn 0099-2240
dc.identifier.uri http://hdl.handle.net/10468/8391
dc.identifier.doi 10.1128/aem.00796-18 en
dc.description.abstract The repertoire of secreted proteins decoded by a microorganism represents proteins released from or associated with the cell surface. In gut commensals, such as bifidobacteria, these proteins are perceived to be functionally relevant, as they regulate the interaction with the gut environment. In the current study, we screened the predicted proteome of over 300 bifidobacterial strains among the currently recognized bifidobacterial species to generate a comprehensive database encompassing bifidobacterial extracellular proteins. A glycobiome analysis of this predicted bifidobacterial secretome revealed that a correlation exists between particular bifidobacterial species and their capability to hydrolyze human milk oligosaccharides (HMOs) and intestinal glycoconjugates, such as mucin. Furthermore, an exploration of metatranscriptomic data sets of the infant gut microbiota allowed the evaluation of the expression of bifidobacterial genes encoding extracellular proteins, represented by ABC transporter substrate-binding proteins and glycoside hydrolases enzymes involved in the degradation of human milk oligosaccharides and mucin. Overall, this study provides insights into how bifidobacteria interact with their natural yet highly complex environment, the infant gut. IMPORTANCE: The ecological success of bifidobacteria relies on the activity of extracellular proteins that are involved in the metabolism of nutrients and the interaction with the environment. To date, information on secreted proteins encoded by bifidobacteria is incomplete and just related to few species. In this study, we reconstructed the bifidobacterial pan-secretome, revealing extracellular proteins that modulate the interaction of bifidobacteria with their natural environment. Furthermore, a survey of the secretion systems between bifidobacterial genomes allowed the identification of a conserved Sec-dependent secretion machinery in all the analyzed genomes and the Tat protein translocation system in the chromosomes of 23 strains belonging to Bifidobacterium longum subsp. longum and Bifidobacterium aesculapii. en
dc.description.sponsorship European Commission (EU Joint Programming Initiative “A Healthy Diet for a Healthy Life” (JPI HDHL; http://www.healthydietforhealthylife.eu/), (in conjunction with Science Fondation Ireland [SFI], grant 15/JP-HDHL/3280)); Fondazione Cariparma (under the TeachInParma Project); en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher American Society for Microbiology en
dc.relation.uri https://aem.asm.org/content/aem/84/16/e00796-18.full.pdf
dc.rights © 2018 American Society for Microbiology. All Rights Reserved. en
dc.subject Bifidobacterium en
dc.subject Bifidobacteria en
dc.subject Genomics en
dc.subject Metagenomics en
dc.subject Secretome en
dc.title Reconstruction of the bifidobacterial pan-secretome reveals the network of extracellular interactions between bifidobacteria and the infant gut en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Douwe Van Sinderen, Microbiology, University College Cork, Cork, Ireland. +353-21-490-3000 Email: d.vansinderen@ucc.ie en
dc.internal.availability Full text available en
dc.date.updated 2019-08-26T13:38:12Z
dc.description.version Published Version en
dc.internal.rssid 497570472
dc.contributor.funder Science Foundation Ireland en
dc.contributor.funder European Commission en
dc.contributor.funder Fondazione Cariparma en
dc.description.status Peer reviewed en
dc.identifier.journaltitle Applied and Environmental Microbiology en
dc.internal.copyrightchecked No
dc.internal.licenseacceptance Yes en
dc.internal.IRISemailaddress d.vansinderen@ucc.ie en
dc.identifier.articleid e00796-18 en
dc.relation.project info:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2273/IE/Alimentary Pharmabiotic Centre (APC) - Interfacing Food & Medicine/ en


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