Biodiversity and classification of phages infecting Lactobacillus brevis
Franz, Charles M. A. P.
van Sinderen, Douwe
Lactobacillus brevis is a lactic acid bacterium that is known as a food and beverage spoilage organism, and more specifically as a beer-spoiler. Phages of L. brevis have been described, but very limited data is available regarding temperate phages of L. brevis. Temperate phages may exert benefits to the host, while they may also be employed to combat beer spoilage. The current study reports on the incidence of prophage sequences present in nineteen distinct L. brevis genomes. Prophage induction was evaluated using mitomycin C exposure followed by genome targeted-PCR, electron microscopy and structural proteome analysis. The morphological and genome sequence analyses revealed significant diversity among L. brevis prophages, which appear to be dominated by members of the Myoviridae phage family. Based on this analysis, we propose a classification of L. brevis phages into five groups.
Lactic acid bacteria , Beer spoilage , Bacteriophage , Lysogeny , Prophage , Genomic analysis , Induction , Myoviridae
Feyereisen, M., Mahony, J., Neve, H., Franz, C. M. A. P., Noben, J.-P., O’Sullivan, T., Boer, V. and van Sinderen, D. (2019) 'Biodiversity and Classification of Phages Infecting Lactobacillus brevis', Frontiers in Microbiology, 10, 2396. (12pp.) DOI: 10.3389/fmicb.2019.02396
© 2019 Feyereisen, Mahony, Neve, Franz, Noben, O’Sullivan, Boer and van Sinderen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.