Biodiversity and classification of phages infecting Lactobacillus brevis
Feyereisen, Marine; Mahony, Jennifer; Neve, Horst; Franz, Charles M. A. P.; Noben, Jean-Paul; O'Sullivan, Tadhg; Boer, Viktor; van Sinderen, Douwe
Date:
2019-10-16
Copyright:
© 2019 Feyereisen, Mahony, Neve, Franz, Noben, O’Sullivan, Boer and van Sinderen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Citation:
Feyereisen, M., Mahony, J., Neve, H., Franz, C. M. A. P., Noben, J.-P., O’Sullivan, T., Boer, V. and van Sinderen, D. (2019) 'Biodiversity and Classification of Phages Infecting Lactobacillus brevis', Frontiers in Microbiology, 10, 2396. (12pp.) DOI: 10.3389/fmicb.2019.02396
Abstract:
Lactobacillus brevis is a lactic acid bacterium that is known as a food and beverage spoilage organism, and more specifically as a beer-spoiler. Phages of L. brevis have been described, but very limited data is available regarding temperate phages of L. brevis. Temperate phages may exert benefits to the host, while they may also be employed to combat beer spoilage. The current study reports on the incidence of prophage sequences present in nineteen distinct L. brevis genomes. Prophage induction was evaluated using mitomycin C exposure followed by genome targeted-PCR, electron microscopy and structural proteome analysis. The morphological and genome sequence analyses revealed significant diversity among L. brevis prophages, which appear to be dominated by members of the Myoviridae phage family. Based on this analysis, we propose a classification of L. brevis phages into five groups.
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Except where otherwise noted, this item's license is described as © 2019 Feyereisen, Mahony, Neve, Franz, Noben, O’Sullivan, Boer and van Sinderen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.