Biodiversity and classification of phages infecting Lactobacillus brevis
Loading...
Files
Published version
Supplementary file 1
Date
2019-10-16
Authors
Feyereisen, Marine
Mahony, Jennifer
Neve, Horst
Franz, Charles M. A. P.
Noben, Jean-Paul
O'Sullivan, Tadhg
Boer, Viktor
van Sinderen, Douwe
Journal Title
Journal ISSN
Volume Title
Publisher
Frontiers Media
Published Version
Abstract
Lactobacillus brevis is a lactic acid bacterium that is known as a food and beverage spoilage organism, and more specifically as a beer-spoiler. Phages of L. brevis have been described, but very limited data is available regarding temperate phages of L. brevis. Temperate phages may exert benefits to the host, while they may also be employed to combat beer spoilage. The current study reports on the incidence of prophage sequences present in nineteen distinct L. brevis genomes. Prophage induction was evaluated using mitomycin C exposure followed by genome targeted-PCR, electron microscopy and structural proteome analysis. The morphological and genome sequence analyses revealed significant diversity among L. brevis prophages, which appear to be dominated by members of the Myoviridae phage family. Based on this analysis, we propose a classification of L. brevis phages into five groups.
Description
Keywords
Lactic acid bacteria , Beer spoilage , Bacteriophage , Lysogeny , Prophage , Genomic analysis , Induction , Myoviridae
Citation
Feyereisen, M., Mahony, J., Neve, H., Franz, C. M. A. P., Noben, J.-P., O’Sullivan, T., Boer, V. and van Sinderen, D. (2019) 'Biodiversity and Classification of Phages Infecting Lactobacillus brevis', Frontiers in Microbiology, 10, 2396. (12pp.) DOI: 10.3389/fmicb.2019.02396